CAJ | 학술논문

目的：克隆并分析胞内劳森氏菌（Lawsonia intracellularis，LI）lsaA基因，为研究该基因提供基础。方法：根据已发表的LI基因组序列，设计2条引物，提取猪回肠黏膜的LI基因组，并用PCR技术扩增lsaA基因，将其克隆、测序，进行序列同源性、蛋白抗原性预测等分析。结果：扩增出与702 bp引物相符的基因片段，与GenBank的序列同源性为98.4%，该基因编码的蛋白预计具有良好的抗原性。结论：胞内劳森菌lasA基因的成功克隆为增生性肠炎的防控提供研究基础。
목적：극륭병분석포내로삼씨균（Lawsonia intracellularis，LI）lsaA기인，위연구해기인제공기출。방법：근거이발표적LI기인조서렬，설계2조인물，제취저회장점막적LI기인조，병용PCR기술확증lsaA기인，장기극륭、측서，진행서렬동원성、단백항원성예측등분석。결과：확증출여702 bp인물상부적기인편단，여GenBank적서렬동원성위98.4%，해기인편마적단백예계구유량호적항원성。결론：포내로삼균lasA기인적성공극륭위증생성장염적방공제공연구기출。
Objective: Cloning and sequence analysis of lsaA gene of Lawsonia intracellularis to lay the foundation for further study of the gene. Methods: 2 primers were designed to amplify Lawsonia intracellularis lsaA gene by PCR. Cloned and sequenced, the nu-cleotide sequence homology of this gene and protein antigenicity prediction analysis were carried out. Results:702 bp PCR product in length were amplified, which have the sequence homology of 98.4% with that of published lsaA gene in Genbank. The protein lsaA encoded by lsaA gene is predicted to have good antigenicity by using DNAStar soft. Conclusion: The successful cloning of Lawsonia intracellularis lsaA gene lay the foundation for the prevention and control of proliferative enteritis.