CAJ | 학술논문

目的：确立大菱鲆的有效麻醉浓度,研究3-氨基苯甲酸乙酯甲基磺酸盐(MS-222)在大菱鲆体内的生物富集和消除规律。方法考察大菱鲆在不同MS-222浓度下麻醉、苏醒过程中的行为变化以及呼吸频率的变化,采用液相色谱-串联质谱法测定大菱鲆组织中的药物水平。结果 MS-222麻醉大菱鲆的有效浓度为60 mg/L,在此浓度下,鱼体能够在5 min之内达到第IV期麻醉状态,5 min之内苏醒恢复。大菱鲆肝脏中MS-222的富集浓度高于肌肉,经48 h消除实验后肌肉和肝脏中的MS-222均低于1μg/kg,其消除半衰期分别为13.14 h(肌肉)和14.15 h(肝脏)。结论在水温不低于(12±0.3)℃条件下,建议将MS-222的休药期定为3天。
목적：학립대릉평적유효마취농도,연구3-안기분갑산을지갑기광산염(MS-222)재대릉평체내적생물부집화소제규률。방법고찰대릉평재불동MS-222농도하마취、소성과정중적행위변화이급호흡빈솔적변화,채용액상색보-천련질보법측정대릉평조직중적약물수평。결과 MS-222마취대릉평적유효농도위60 mg/L,재차농도하,어체능구재5 min지내체도제IV기마취상태,5 min지내소성회복。대릉평간장중MS-222적부집농도고우기육,경48 h소제실험후기육화간장중적MS-222균저우1μg/kg,기소제반쇠기분별위13.14 h(기육)화14.15 h(간장)。결론재수온불저우(12±0.3)℃조건하,건의장MS-222적휴약기정위3천。
ABSTRACT:Objective Toestablish effective anesthesia concentrations and study the regularity of accu-mulation and elimination of tricaine methanesulfonate (MS-222) in muscle and liver of turbot. Methods Under different concentrations of MS-222 anesthesia, waking up in the process of behavior change and the change of respiratory frequency were studied in turbot. The residues were detected by liquid chromatogra-phy-tandem mass spectrometry (LC-MS/MS).ResultsThe effective concentration of MS-222 for turbot was 60 mg/L, and under this condition, it reached stage IV anesthesia and recovered within 5 min. The ac-cumulation amount of turbot liver was higher than that of the muscle. The MS-222 concentration was elimi-nated below safe quantity (1μg/kg) in muscle and liver after 48 h, and the elimination half-life were 13.14 h (muscle) and 14.15 h (liver).ConclusionUnder the condition of this experiment, it was recommended withdrawal period of 3 d.