CAJ | 학술논문

目的：在已制备出抗一类有机磷农药的组特异性单克隆抗体的基础上,制备胶体金,从而快速地对上述有机磷农药进行检测。方法通过鞣酸-柠檬酸三钠还原法制备出直径16.08±0.64 nm、质地均一的胶体金,并且以此标记单克隆抗体,组装胶体金免疫层析检测板。利用紫外光谱、荧光光谱、透射电镜、动态光散射等方法对胶体金及金标抗体进行鉴定。结果该检测板对标准毒死蜱的检测限为4μg/mL,检测时间为5 min。结论胶体金试纸条对毒死蜱具有较好的检测灵敏度,并且基质的干扰效应不明显。
목적：재이제비출항일류유궤린농약적조특이성단극륭항체적기출상,제비효체금,종이쾌속지대상술유궤린농약진행검측。방법통과유산-저몽산삼납환원법제비출직경16.08±0.64 nm、질지균일적효체금,병차이차표기단극륭항체,조장효체금면역층석검측판。이용자외광보、형광광보、투사전경、동태광산사등방법대효체금급금표항체진행감정。결과해검측판대표준독사비적검측한위4μg/mL,검측시간위5 min。결론효체금시지조대독사비구유교호적검측령민도,병차기질적간우효응불명현。
ABSTRACT:Objective To prepare the colloidal gold for the rapid detection of organicphosphorus pesti-cides on the basis of the specific monoclonal antibodies against a class of organicphosphorus pesticides.Me-thods Uniform texture colloidal gold with a diameter of 16.08+0.64 nm was prepared by tannin-sodium ci-trate reduction method, then labeled monoclonal antibody, and assembled colloidal gold immune chromato-graphy testing board. The colloidal gold and gold labeled antibodies were identified by ultraviolet spectrum, fluorescence spectroscopy, transmission electron microscopy, and dynamic light scattering.Results The test-ing board was validated to complete a detection assay within 5 min, and the LOD value of chlorpyriphos-ethyl was 4μg/mL.ConclusionColloidal gold test strip had a high sensitivity, and the matrix interference effect was not obvious.