CAJ | 학술논문

目的：制备和筛选出一种能识别一类或几类具有结构相似的有机磷杀虫剂的广谱性鼠单克隆抗体(mAb)以对多农药残留同时进行检测。方法制备抗有机磷组特异性单克隆抗体,利用间接竞争性ELISA法对几种有机磷农药的标准品进行检测。结果对乙基毒死蜱检测灵敏度较高,最低检测限(LOD)为4 ng/mL,对甲基对硫磷的LOD为63 ng/mL；辛硫磷和三唑磷的检测敏感度低、一致性差。结论根据农药化学结构的不同,检测灵敏度有一定的差别,对乙基毒死蜱和甲基对硫磷检测效果较好。
목적：제비화사선출일충능식별일류혹궤류구유결구상사적유궤린살충제적엄보성서단극륭항체(mAb)이대다농약잔류동시진행검측。방법제비항유궤린조특이성단극륭항체,이용간접경쟁성ELISA법대궤충유궤린농약적표준품진행검측。결과대을기독사비검측령민도교고,최저검측한(LOD)위4 ng/mL,대갑기대류린적LOD위63 ng/mL；신류린화삼서린적검측민감도저、일치성차。결론근거농약화학결구적불동,검측령민도유일정적차별,대을기독사비화갑기대류린검측효과교호。
ABSTRACT:Objective To prepare and screen a broad spectrum of monoclonal antibodies (mAb), which can identify a class of organophosphorus pesticide with similar structures for the simultaneous detection of multiple pesticide residues. MethodsMonoclonal antibodies against organophosphorus pesticidewere pre-pared. Several standard of organophosphorus pesticide were detected by indirect competitive ELISA.Results The limit of detecting (LOD) value of chlorpyriphos-ethyl was 4 ng/mL with high sensitivity. The LOD value for parathion-merhyl was 63 ng/mL. The sensitivity of the detection of the phoxim and phentriazophos was low. ConclusionThe sensitivity of detection was distincted because of the difference of chemical structure. The detection effect of chlorpyrifos-ethyl and parathion-methyl was good.