国际眼科杂志
國際眼科雜誌
국제안과잡지
International Eye Science
2015年
12期
2060-2062
,共3页
糖尿病视网膜病变%结缔组织生长因子%糖尿病大鼠
糖尿病視網膜病變%結締組織生長因子%糖尿病大鼠
당뇨병시망막병변%결체조직생장인자%당뇨병대서
diabetic retinopathy%connective tissue growth factor%diabetic rats
目的：探讨结缔组织生长因子（ connective tissue growth factor，CTGF）对大鼠糖尿病视网膜病变的影响及复方血栓通可能的保护机制。<br> 方法：SD大鼠36只随机分为对照组（ A组，n＝12）和造模组（n＝24），造模组注射链脲佐菌素（STZ）60mg／kg，造模成功后随即分为糖尿病组（ B组，n＝12）、复方血栓通干预组[C组，1g／（kg· d），n＝12]，于实验第6、12wk分别在各组处死6只大鼠，RT－PCR方法检测视网膜组织中CTGF mRNA水平。<br> 结果：与A组相比，B组、C组大鼠视网膜CTGF mRNA于第6、12wk 表达均增加（均 P＜0．05），C 组视网膜组织CTGF mRNA表达于第6、12wk 均较 B 组明显下调（ P＜0.05）；电镜显示B组大鼠视网膜毛细血管内皮细胞肿胀，胞质内线粒体肿胀，吞饮小泡增多，毛细血管基底膜电子密度不均匀，有结节样增厚现象，C组视网膜病变较B组减轻。<br> 结论：CTGF可能参与糖尿病视网膜病变发生，复方血栓通对糖尿病大鼠视网膜具有保护作用，可能通过下调CTGF mRNA产生作用。
目的：探討結締組織生長因子（ connective tissue growth factor，CTGF）對大鼠糖尿病視網膜病變的影響及複方血栓通可能的保護機製。<br> 方法：SD大鼠36隻隨機分為對照組（ A組，n＝12）和造模組（n＝24），造模組註射鏈脲佐菌素（STZ）60mg／kg，造模成功後隨即分為糖尿病組（ B組，n＝12）、複方血栓通榦預組[C組，1g／（kg· d），n＝12]，于實驗第6、12wk分彆在各組處死6隻大鼠，RT－PCR方法檢測視網膜組織中CTGF mRNA水平。<br> 結果：與A組相比，B組、C組大鼠視網膜CTGF mRNA于第6、12wk 錶達均增加（均 P＜0．05），C 組視網膜組織CTGF mRNA錶達于第6、12wk 均較 B 組明顯下調（ P＜0.05）；電鏡顯示B組大鼠視網膜毛細血管內皮細胞腫脹，胞質內線粒體腫脹，吞飲小泡增多，毛細血管基底膜電子密度不均勻，有結節樣增厚現象，C組視網膜病變較B組減輕。<br> 結論：CTGF可能參與糖尿病視網膜病變髮生，複方血栓通對糖尿病大鼠視網膜具有保護作用，可能通過下調CTGF mRNA產生作用。
목적：탐토결체조직생장인자（ connective tissue growth factor，CTGF）대대서당뇨병시망막병변적영향급복방혈전통가능적보호궤제。<br> 방법：SD대서36지수궤분위대조조（ A조，n＝12）화조모조（n＝24），조모조주사련뇨좌균소（STZ）60mg／kg，조모성공후수즉분위당뇨병조（ B조，n＝12）、복방혈전통간예조[C조，1g／（kg· d），n＝12]，우실험제6、12wk분별재각조처사6지대서，RT－PCR방법검측시망막조직중CTGF mRNA수평。<br> 결과：여A조상비，B조、C조대서시망막CTGF mRNA우제6、12wk 표체균증가（균 P＜0．05），C 조시망막조직CTGF mRNA표체우제6、12wk 균교 B 조명현하조（ P＜0.05）；전경현시B조대서시망막모세혈관내피세포종창，포질내선립체종창，탄음소포증다，모세혈관기저막전자밀도불균균，유결절양증후현상，C조시망막병변교B조감경。<br> 결론：CTGF가능삼여당뇨병시망막병변발생，복방혈전통대당뇨병대서시망막구유보호작용，가능통과하조CTGF mRNA산생작용。
?AIM:To explore the effects of connective tissue growth factor( CTGF) on diabetic retinopathy ( DR) in rats,and the potential protective mechanism of compound xueshuantong to DR. <br> ?METHODS:Sprague-Dawley( SD) rats were divided into normal group(group a,n=12)and model group(n=24). Rats in model group received intraperitoneal injection of streptozotocin(60mg/kg body weight) to get diabetic rats, which were randomly subdivided into diabetic group ( group B, n=12 ) and xueshuantong intervention group [group C,1g/(kg· d),n=12].At 6th and 12th wk of the experiment, 6 rats randomly selected from every group, were executed to get retinal tissue for detecting CTGF-mRNA by electron microscope. <br> ?RESULTS:Compared with group A,the content of CTGF-mRNA in retina tissue increased at 6, 12wk in both group B and C (P<0.05).Compared with group B,the content of CTGF-mRNA in retina tissue in group C decreased at 6, 12wk(P<0.05).Electron microscope showed:in group B, the capillary endothelial cells and mitochondria became swollen;the amount of pinocytosis vesicle increased;the electron density in capillary basement membrane was not homogeneous and nodular thickening phenomenon appeared;compared with group B,the changes in group C were alleviated. <br> ?CONCLUSION:CTGF may be involved in the progress of DR in diabetic rats.Compound xueshuantong may exert its protective effect through decreasing the expression of CTGF in the retina of diabetic rats.
