国际眼科杂志
國際眼科雜誌
국제안과잡지
International Eye Science
2015年
12期
2045-2048
,共4页
miR-218%视网膜母细胞瘤%增殖%凋亡%Bmi-1
miR-218%視網膜母細胞瘤%增殖%凋亡%Bmi-1
miR-218%시망막모세포류%증식%조망%Bmi-1
miR -218%retinoblastoma%proliferation%apoptosis%Bmi-1
目的：探讨miR－218在人视网膜母细胞瘤中的表达、临床意义及其分子机制。<br> 方法：实时定量PCR检测miR－218在视网膜母细胞瘤及瘤旁组织中的表达，并分析其与临床病理特征的关系。检测miR－218在视网膜母细胞瘤细胞系中的表达，并用miR－218模拟物转染Y79细胞，MTT及流式细胞仪检测Y79细胞的增殖、凋亡情况，RT－PCR和Western－blot法检测Bmi－1 mRNA及蛋白的表达。<br> 结果：miR－218在视网膜母细胞瘤组织中的表达水平显著低于对应瘤旁组织；miR－218低表达与神经浸润、分化程度密切相关；miR－218可显著抑制Y79细胞的增殖并促进其凋亡，并下调Bmi－1的mRNA及蛋白表达水平。<br> 结论：miR－218在人视网膜母细胞瘤组织中表达下调，并与临床病理相关，miR－218抑制Y79细胞增殖、促进凋亡的作用可能与下调Bmi－1有关。
目的：探討miR－218在人視網膜母細胞瘤中的錶達、臨床意義及其分子機製。<br> 方法：實時定量PCR檢測miR－218在視網膜母細胞瘤及瘤徬組織中的錶達，併分析其與臨床病理特徵的關繫。檢測miR－218在視網膜母細胞瘤細胞繫中的錶達，併用miR－218模擬物轉染Y79細胞，MTT及流式細胞儀檢測Y79細胞的增殖、凋亡情況，RT－PCR和Western－blot法檢測Bmi－1 mRNA及蛋白的錶達。<br> 結果：miR－218在視網膜母細胞瘤組織中的錶達水平顯著低于對應瘤徬組織；miR－218低錶達與神經浸潤、分化程度密切相關；miR－218可顯著抑製Y79細胞的增殖併促進其凋亡，併下調Bmi－1的mRNA及蛋白錶達水平。<br> 結論：miR－218在人視網膜母細胞瘤組織中錶達下調，併與臨床病理相關，miR－218抑製Y79細胞增殖、促進凋亡的作用可能與下調Bmi－1有關。
목적：탐토miR－218재인시망막모세포류중적표체、림상의의급기분자궤제。<br> 방법：실시정량PCR검측miR－218재시망막모세포류급류방조직중적표체，병분석기여림상병리특정적관계。검측miR－218재시망막모세포류세포계중적표체，병용miR－218모의물전염Y79세포，MTT급류식세포의검측Y79세포적증식、조망정황，RT－PCR화Western－blot법검측Bmi－1 mRNA급단백적표체。<br> 결과：miR－218재시망막모세포류조직중적표체수평현저저우대응류방조직；miR－218저표체여신경침윤、분화정도밀절상관；miR－218가현저억제Y79세포적증식병촉진기조망，병하조Bmi－1적mRNA급단백표체수평。<br> 결론：miR－218재인시망막모세포류조직중표체하조，병여림상병리상관，miR－218억제Y79세포증식、촉진조망적작용가능여하조Bmi－1유관。
?AIM:To investigate the level of miR-218 in human retinoblastoma ( RB ) and its effect on the potential mechanism of tumorgenesis. <br> ?METHODS:Real-time PCR was applied to detect the expression of miR -218 in human RB and the corresponding tumor -adjacent tissues to analyze the relation between miR -218 and clinic pathology characteristics. The artificial miR -218 was transiently transfected into human Y79 cells in vitro.The proliferation and apoptosis of the cells were detected by MTT assay and flow cytometry, respectively.The expression level of Bmi-1 mRNA and protein were determined by RT-PCR and Western blot. <br> ?RESULTS:The expression level of miR-218 in RB tissues was significantly lower than that in the adjacent tissues, and it was associated with optic nerve infiltration and differentiated degree.Over-expressed miR-218 in Y79 cells suppressed cell proliferation and promoted cell apoptosis, and down -regulated mRNA and protein expressions of Bmi-1. <br> ?CONCLUSION:The expression of miR-218 in RB tissues is significantly lower than that in tumor-adjacent tissues. MiR-218 could inhibit RB cell proliferation and induce apoptosis by down-regulating the expression of Bmi-1.
