CAJ | 학술논문

目的：筛选并鉴定唐氏综合征胎儿与健康对照者差异性表达蛋白质，以寻找唐氏综合征胎儿的生物标记物。方法应用同位素标记相对和绝对蛋白定量法和功能聚类分析法筛查和分析唐氏综合征胎儿脐带血和正常胎儿脐带血的差异表达蛋白。结果共筛选鉴定到506种差异表达蛋白，其中最显著的3种蛋白分别是基质蛋白‐3、胸腺肽β10和骨桥蛋白。结论唐氏综合征胎儿和正常胎儿脐带血血清蛋白存在较多差异表达蛋白，其中基质蛋白‐3、胸腺肽β10和骨桥蛋白等显著差异蛋白可能作为潜在的临床筛查生物标志物。
목적：사선병감정당씨종합정태인여건강대조자차이성표체단백질，이심조당씨종합정태인적생물표기물。방법응용동위소표기상대화절대단백정량법화공능취류분석법사사화분석당씨종합정태인제대혈화정상태인제대혈적차이표체단백。결과공사선감정도506충차이표체단백，기중최현저적3충단백분별시기질단백‐3、흉선태β10화골교단백。결론당씨종합정태인화정상태인제대혈혈청단백존재교다차이표체단백，기중기질단백‐3、흉선태β10화골교단백등현저차이단백가능작위잠재적림상사사생물표지물。
Objective To identify the differentially expressed proteins between fetuses with Down′s syndrome and healthy control ,so as to screen the potential biomarkers of fetuses with Down′s syndrome .Methods To investi‐gate the differentially expressed proteins in cord blood of fetus with Down′s syndrome and normal fetus using isobaric tagging for relative and absolute protein quantification and gene gene ontology analysis .Results A total of 506 dif‐ferentiate proteins were identified ,in which 3 proteins (Matrin‐3 ,Thymosin beta 10 and Osteopontin) were signifi‐cant .Conclusion The proteomics of the serum are significant different among normal fetus and fetus with Down′s syndrome .Some of the protein ,such as Matrin‐3 ,Thymosin beta 10 and Osteopontin ,may have potential applying value in clinic screening pratice .