临床荟萃
臨床薈萃
림상회췌
Clinical Focus
2015年
12期
1421-1423,1426
,共4页
席素雅%张玲%张新%王少颖
席素雅%張玲%張新%王少穎
석소아%장령%장신%왕소영
哮喘%白细胞介素 13%基因多态性%限制性片断长度
哮喘%白細胞介素 13%基因多態性%限製性片斷長度
효천%백세포개소 13%기인다태성%한제성편단장도
asthma%interleukin-13%gene polymorphism%restriction fragment length
目的：探讨白细胞介素13(IL-13)基因第3内含子+1923C/T 及第4外显子+2044G/A 多态性与哮喘的关系。方法采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)方法,检测100例哮喘患者和100例健康人群 IL-13基因+1923C/T 及+2044G/A 位点单核苷酸多态性,分析其基因型和等位基因分布频率。结果 IL-13+1923C/T 位点基因型 CC、CT 和 TT 在哮喘组分布频率为21.0%、41.0%和38.0%,对照组为41.0%、44.0%和15.0%。IL-13+2044G/A 位点基因型 GG、GA 和 AA 在哮喘组分布频率为51.0%、39.0%和10.0%,对照组为70.0%、25.0%和5.0%。IL-13+1923C/T 和+2044G/A 位点各基因型分布在两组间差异有统计学意义(χ2=16.54,P <0.01；χ2=7.71,P <0.05)。结论 IL-13基因+1923C/T 和+2044G/A 多态性与哮喘易感性相关,携带+1923T 或+2044A 等位基因的个体患哮喘的风险更大。
目的：探討白細胞介素13(IL-13)基因第3內含子+1923C/T 及第4外顯子+2044G/A 多態性與哮喘的關繫。方法採用聚閤酶鏈反應限製性片段長度多態性(PCR-RFLP)方法,檢測100例哮喘患者和100例健康人群 IL-13基因+1923C/T 及+2044G/A 位點單覈苷痠多態性,分析其基因型和等位基因分佈頻率。結果 IL-13+1923C/T 位點基因型 CC、CT 和 TT 在哮喘組分佈頻率為21.0%、41.0%和38.0%,對照組為41.0%、44.0%和15.0%。IL-13+2044G/A 位點基因型 GG、GA 和 AA 在哮喘組分佈頻率為51.0%、39.0%和10.0%,對照組為70.0%、25.0%和5.0%。IL-13+1923C/T 和+2044G/A 位點各基因型分佈在兩組間差異有統計學意義(χ2=16.54,P <0.01；χ2=7.71,P <0.05)。結論 IL-13基因+1923C/T 和+2044G/A 多態性與哮喘易感性相關,攜帶+1923T 或+2044A 等位基因的箇體患哮喘的風險更大。
목적：탐토백세포개소13(IL-13)기인제3내함자+1923C/T 급제4외현자+2044G/A 다태성여효천적관계。방법채용취합매련반응한제성편단장도다태성(PCR-RFLP)방법,검측100례효천환자화100례건강인군 IL-13기인+1923C/T 급+2044G/A 위점단핵감산다태성,분석기기인형화등위기인분포빈솔。결과 IL-13+1923C/T 위점기인형 CC、CT 화 TT 재효천조분포빈솔위21.0%、41.0%화38.0%,대조조위41.0%、44.0%화15.0%。IL-13+2044G/A 위점기인형 GG、GA 화 AA 재효천조분포빈솔위51.0%、39.0%화10.0%,대조조위70.0%、25.0%화5.0%。IL-13+1923C/T 화+2044G/A 위점각기인형분포재량조간차이유통계학의의(χ2=16.54,P <0.01；χ2=7.71,P <0.05)。결론 IL-13기인+1923C/T 화+2044G/A 다태성여효천역감성상관,휴대+1923T 혹+2044A 등위기인적개체환효천적풍험경대。
ABSTRACT:Objective To explore the correlation between single nucleotide polymorphisms (SNPs)+ 1 923C/T in intron3 region and + 2044G/A in exon4 region of interleukin-13 (IL-13 )gene and the susceptibility of asthma. Methods A total of 100 asthmatic patients and 100 healthy controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)methods.The differences of genotype and allele frequency between the two groups were detected and analyzed.Results The genotype frequencies of CC,CT and TT in IL-13 +1 923C/T site were 21.0%,41.0% and 38.0% in the asthmatic subjects and 41.0%,44.0% and 1 5.0% in the controls,respectively.The genotype frequencies of GG,GA and AA in + 2044G/A site were 5 1.0%,39.0% and 10.0% in the asthmatic subjects and 70.0%,25.0% and 5.0% in the controls,respectively.The distribution of genotype in +1 923C/T and +2044G/A sites of IL-13 gene between the two groups were significantly different (χ2 =1 6.54,P <0.01;χ2 =7.71,P <0.05,respectively).Conclusion The SNPs of +1 923C/T and +2044G/A in IL-13 gene were associated with the susceptibility to asthma.Subjects carrying the + 1 923T allele or + 2044A allele may have an increased risk of asthma.
