东北林业大学学报
東北林業大學學報
동북임업대학학보
Journal of Northeast Forestry University
2015年
11期
22-27
,共6页
廖源林%蔡仕珍%邓辉茗%龙聪颖%陈彦青%叶充%熊磊%王琰琳
廖源林%蔡仕珍%鄧輝茗%龍聰穎%陳彥青%葉充%熊磊%王琰琳
료원림%채사진%산휘명%룡총영%진언청%협충%웅뢰%왕염림
苦楝%Cd2+胁迫%抗氧化酶%AsA-GSH循环
苦楝%Cd2+脅迫%抗氧化酶%AsA-GSH循環
고련%Cd2+협박%항양화매%AsA-GSH순배
Melia azedarach Linn.%Cadmium stress%Antioxidant enzymes%AsA-GSH cycle
以1年生苦楝幼苗为试材,研究含重金属Cd2+质量分数分别为30、60、90、120、150、180 mg·kg-1的土壤对苦楝叶片SOD、CAT、POD活性及抗坏血酸—谷胱甘肽( AsA-GSH)循环系统的影响。结果表明:处理时间20、40 d,Cd2+质量分数分别为30、60、90 mg·kg-1时,以及处理时间60 d,Cd2+质量分数分别为30、60 mg·kg-1时,苦楝的SOD、CAT、POD活性大幅度提高,AsA-GSH循环系统的抗氧化剂抗坏血酸( ASA)质量分数和谷胱甘肽( GSH)质量摩尔浓度,以及相关酶抗坏血酸过氧化物酶( APX)活性、脱氢抗坏血酸还原酶( DHAR)活性和谷胱甘肽还原酶(GR)活性均呈增加趋势,抗氧化系统高效运行;处理时间为20、40 d,Cd2+质量分数分别为90、120 mg·kg-1时,以及处理时间为60 d,Cd2+质量分数分别为60、90 mg·kg-1时,SOD、CAT、POD活性及AsA-GSH循环的抗氧化剂和相关酶活性的变化趋势不尽一致,表明整个抗氧化系统具有协同清除活性氧的作用;处理时间为60 d,Cd2+质量分数为180 mg·kg-1时,CAT和POD活性以及AsA-GSH循环中的APX、DHAR活性依然维持着明显高于CK的水平,表明苦楝的抗氧化系统没有被高质量分数的Cd2+胁迫摧毁,但受到较大程度抑制。综上认为,苦楝对Cd2+胁迫具有较强的耐受能力,对Cd2+胁迫的耐受范围大约为20 d和40 d处理下Cd2+质量分数低于150 mg·kg-1,60 d处理下低于120 mg·kg-1。
以1年生苦楝幼苗為試材,研究含重金屬Cd2+質量分數分彆為30、60、90、120、150、180 mg·kg-1的土壤對苦楝葉片SOD、CAT、POD活性及抗壞血痠—穀胱甘肽( AsA-GSH)循環繫統的影響。結果錶明:處理時間20、40 d,Cd2+質量分數分彆為30、60、90 mg·kg-1時,以及處理時間60 d,Cd2+質量分數分彆為30、60 mg·kg-1時,苦楝的SOD、CAT、POD活性大幅度提高,AsA-GSH循環繫統的抗氧化劑抗壞血痠( ASA)質量分數和穀胱甘肽( GSH)質量摩爾濃度,以及相關酶抗壞血痠過氧化物酶( APX)活性、脫氫抗壞血痠還原酶( DHAR)活性和穀胱甘肽還原酶(GR)活性均呈增加趨勢,抗氧化繫統高效運行;處理時間為20、40 d,Cd2+質量分數分彆為90、120 mg·kg-1時,以及處理時間為60 d,Cd2+質量分數分彆為60、90 mg·kg-1時,SOD、CAT、POD活性及AsA-GSH循環的抗氧化劑和相關酶活性的變化趨勢不儘一緻,錶明整箇抗氧化繫統具有協同清除活性氧的作用;處理時間為60 d,Cd2+質量分數為180 mg·kg-1時,CAT和POD活性以及AsA-GSH循環中的APX、DHAR活性依然維持著明顯高于CK的水平,錶明苦楝的抗氧化繫統沒有被高質量分數的Cd2+脅迫摧燬,但受到較大程度抑製。綜上認為,苦楝對Cd2+脅迫具有較彊的耐受能力,對Cd2+脅迫的耐受範圍大約為20 d和40 d處理下Cd2+質量分數低于150 mg·kg-1,60 d處理下低于120 mg·kg-1。
이1년생고련유묘위시재,연구함중금속Cd2+질량분수분별위30、60、90、120、150、180 mg·kg-1적토양대고련협편SOD、CAT、POD활성급항배혈산—곡광감태( AsA-GSH)순배계통적영향。결과표명:처리시간20、40 d,Cd2+질량분수분별위30、60、90 mg·kg-1시,이급처리시간60 d,Cd2+질량분수분별위30、60 mg·kg-1시,고련적SOD、CAT、POD활성대폭도제고,AsA-GSH순배계통적항양화제항배혈산( ASA)질량분수화곡광감태( GSH)질량마이농도,이급상관매항배혈산과양화물매( APX)활성、탈경항배혈산환원매( DHAR)활성화곡광감태환원매(GR)활성균정증가추세,항양화계통고효운행;처리시간위20、40 d,Cd2+질량분수분별위90、120 mg·kg-1시,이급처리시간위60 d,Cd2+질량분수분별위60、90 mg·kg-1시,SOD、CAT、POD활성급AsA-GSH순배적항양화제화상관매활성적변화추세불진일치,표명정개항양화계통구유협동청제활성양적작용;처리시간위60 d,Cd2+질량분수위180 mg·kg-1시,CAT화POD활성이급AsA-GSH순배중적APX、DHAR활성의연유지착명현고우CK적수평,표명고련적항양화계통몰유피고질량분수적Cd2+협박최훼,단수도교대정도억제。종상인위,고련대Cd2+협박구유교강적내수능력,대Cd2+협박적내수범위대약위20 d화40 d처리하Cd2+질량분수저우150 mg·kg-1,60 d처리하저우120 mg·kg-1。
By using annual Melia azedarach seedlings as test materials, we studied the effects of heavy metal Cd2+( 0, 30, 60, 90, 120, 150, and 180 mg·kg-1 ) stress on SOD, CAT, POD activity and ascorbate glutathione ( ASA-GSH) circulation system of M. azedarach leaf. When the treating time were 20 and 40 days with Cd2+contents of 30 mg·kg-1 , 60 mg·kg-1 and 90 mg·kg-1 , and the treating time was 60 days with Cd2+ contents of 30 mg·kg-1 and 60 mg·kg-1 , the activity of SOD, CAT and POD of M. azedarach were improved greatly, the content of AsA and GSH of AsA-GSH cycle and related enzyme activities of APX, DHAR and GR showed increasing trend, and antioxidant system operated efficiently. When the treating time were 20 days and 40 days with Cd2+contents of 90 mg·kg-1 and 120 mg·kg-1 , and the treating time was 60 days with the Cd2+contents of 60 mg·kg-1 and 90 mg·kg-1 , the activity of SOD, CAT and POD and antioxidants and en?zymes of AsA-GSH cycle had different changing trends, which showed that the antioxidant system played a synergistic role in scavenging reactive oxygen. When the treating time was 60 days with Cd2+content of 180 mg·kg-1 , the activity of CAT and POD and the content of APX and DHAR of AsA-GSH cycle still maintain a level, significantly higher than that of CK. The antioxidant system of M. azedarach was not destroyed by stress of high concentrations of Cd2+, but was the inhibited greatly. M. azedarach has a strong ability of tolerance for Cd stress, and the range of tolerance is: the treating time is 20 days and 40 days with the Cd2+contents of less than 150 mg·kg-1 , or when the treating time is 60 days with Cd2+contents of less than 120 mg·kg-1 , respectively.