CAJ | 학술논문

以7日龄刺槐实生苗的下胚轴作为外植体进行离体培养，研究不同生长调节剂、下胚轴部位和接种方式对不定芽分化的影响。结果表明：不同质量浓度的6－BA和NAA及组合对刺槐下胚轴的不定芽分化具有较大影响；不同部位的下胚轴再生不定芽的能力存在显著差异；下胚轴在培养基中的接种方式对不定芽的分化具有一定影响；下胚轴再生具有极性现象。刺槐下胚轴不定芽分化的最佳培养基为MS＋6－BA1．5 mg · L－1＋NAA0．05 mg·L－1＋3％蔗糖，最佳外植体为靠近胚根端的下胚轴部分，接种方式为水平接种，其不定芽诱导率最高，达68．52％，平均诱导不定芽数为5．56个；诱导不定芽生根的最佳培养基为MS＋IBA0．20 mg·L－1＋3％蔗糖，封口日光驯化1周后，以V（珍珠岩）∶ V（泥炭土）∶ V（蛭石）＝1∶1∶1为基质移栽，生根苗经移栽成活率为100％。
이7일령자괴실생묘적하배축작위외식체진행리체배양，연구불동생장조절제、하배축부위화접충방식대불정아분화적영향。결과표명：불동질량농도적6－BA화NAA급조합대자괴하배축적불정아분화구유교대영향；불동부위적하배축재생불정아적능력존재현저차이；하배축재배양기중적접충방식대불정아적분화구유일정영향；하배축재생구유겁성현상。자괴하배축불정아분화적최가배양기위MS＋6－BA1．5 mg · L－1＋NAA0．05 mg·L－1＋3％자당，최가외식체위고근배근단적하배축부분，접충방식위수평접충，기불정아유도솔최고，체68．52％，평균유도불정아수위5．56개；유도불정아생근적최가배양기위MS＋IBA0．20 mg·L－1＋3％자당，봉구일광순화1주후，이V（진주암）∶ V（니탄토）∶ V（질석）＝1∶1∶1위기질이재，생근묘경이재성활솔위100％。
We studied the effects of different plant growth regulators, the position and inoculation method of hypocotyl segments on adventitious shoots induction and plant regeneration using hypocotyl of seven?day?old seedlings of Robinia pseudoacacia L. as explants. Different parts of explants and the concentrations and ration of 6-BA to NAA highly influenced the adventi?tious shoots regeneration. The capability of generation of adventitious shoots highly varied among the hypocotyl in different position. The type of inoculatiom also highly influenced adventitious shoots induction. There was polarity in the process of hypocotyl regeneration. The highest regeneration rate ( 68. 52%) was obtained when using hypocotyl near the radical, placed horizontally and cultured on MS basal medium supplemented with 1.5 mg·L-1 6-BA, 0.05 mg·L-1 NAA and 3%sugar. An average of 5.56 shoots per explant was obtained. The most suitable medium for root regeneration were on MS bas?al medium supplemented with 0.20 mg·L-1 IBA and 3% sugar. After the close lid in sunlight for seven days, and train seedlings were transferred into the mixture with the ratio of perlite, peat soil and vermiculite 1 ∶ 1 ∶ 1, and 100% of them were successfully acclimatized.