CAJ | 학술논문

采用农杆菌介导法将毛果杨（ Populus trichocarpa） PGR5基因转化南林895杨，经潮霉素抗性基因筛选并对转基因植株进行了分子检测和表达量分析。普通PCR检测结果显示，有12株检测到阳性信号，表明该基因已整合到南林895杨基因组中；经RT－PCR检测，该12株转化子在目的基因处有阳性条带，表明该基因在转录水平表达；实时荧光定量PCR检测结果显示，12株阳性苗的表达量为野生型的2～20倍。
채용농간균개도법장모과양（ Populus trichocarpa） PGR5기인전화남림895양，경조매소항성기인사선병대전기인식주진행료분자검측화표체량분석。보통PCR검측결과현시，유12주검측도양성신호，표명해기인이정합도남림895양기인조중；경RT－PCR검측，해12주전화자재목적기인처유양성조대，표명해기인재전록수평표체；실시형광정량PCR검측결과현시，12주양성묘적표체량위야생형적2～20배。
We introduced PGR5 gene from Populus trichocarpa to Populus×euramericana cv. ‘Nanlin895 by agrobacterium medi?ated method, screened these transgenic plants by hygromycin resistance gene, and took the molecular detection and expres?sion analysis of these transgenic plants. With the data of PCR detection, 12 of 23 clones were positives, and the gene was integrated into the genome of poplars. By RT?PCR detection, 12 clones had positive bands which were consistent with the anticipated objective strap size, and the gene was expressed at the transcripional level. By real time?PCR detection, the ex?pression of the 12 positive clones were 220 times as much as wild types.