CAJ | 학술논문

为了探明松材线虫（ Bursaphelenchus xylophilus）与拟松材线虫（ Bursaphelenchus mucronatus）能否进行种间杂交，收集了不同地理来源的松材线虫和拟松材线虫株系，在实验室内进行了种间杂交、继代培养以及回交。结果显示：中国浙江松材线虫和法国拟松材线虫、中国浙江松材线虫和中国拟松材线虫杂交组合，具有较高的交配率、繁殖量和低死亡率。在继代培养试验中，这2个组合都具有很强的繁殖优势，其杂交后代可以同亲本回交，且更趋于同拟松材线虫回交。总的来说，松材线虫和拟松材线虫有些虫株间是可以杂交并能够顺利繁殖，建立可延续的种群的。推测在自然条件下，松材线虫和拟松材线虫杂交后代是存在的。
위료탐명송재선충（ Bursaphelenchus xylophilus）여의송재선충（ Bursaphelenchus mucronatus）능부진행충간잡교，수집료불동지리래원적송재선충화의송재선충주계，재실험실내진행료충간잡교、계대배양이급회교。결과현시：중국절강송재선충화법국의송재선충、중국절강송재선충화중국의송재선충잡교조합，구유교고적교배솔、번식량화저사망솔。재계대배양시험중，저2개조합도구유흔강적번식우세，기잡교후대가이동친본회교，차경추우동의송재선충회교。총적래설，송재선충화의송재선충유사충주간시가이잡교병능구순리번식，건립가연속적충군적。추측재자연조건하，송재선충화의송재선충잡교후대시존재적。
In our study different isolates of Bursaphelenchus xylophilus and B. mucronatus were collected from different origins. Interspecific hybridization, successive transfer culture and backcross test were conducted in the laboratory. The combina?tions of B. xylophilus from Zhejiang hybridizes and B. mucronatus from France or B. mucronatus from China had high hybrid rate, large number of descendants, and low death rate. In subculture tests, both combinations had a strong reproductive advantage. The hybrid offspring of two combinations could cross with parents, especially the B. mucronatus. Therefore, there is interspecific hybridization between some B. xylophilus and B. mucronatus actually. The hybrids can establish stable population, and there is the hybrid offspring of B. xylophilus and B. mucronatus exist in natural conditions.