食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
Journal of Food Safety & Quality
2015年
11期
4336-4340
,共5页
张朝晖%李建辉%崔凤云%何悦%别玮%韩深%刘韦华%刘鑫%严华%齐鹤鸣
張朝暉%李建輝%崔鳳雲%何悅%彆瑋%韓深%劉韋華%劉鑫%嚴華%齊鶴鳴
장조휘%리건휘%최봉운%하열%별위%한심%류위화%류흠%엄화%제학명
乳品%左旋肉碱%高效液相色谱-质谱联用法
乳品%左鏇肉堿%高效液相色譜-質譜聯用法
유품%좌선육감%고효액상색보-질보련용법
milk products%L-carnitine%high performance liquid chromatography-tandem mass spectrometry
目的：建立高效液相色谱串联质谱法测定乳品中左旋肉碱的含量。方法样品中的左旋肉碱用乙腈水(3：1, V：V)溶液提取,提取液经碱皂化后使溶液中结合态的左旋肉碱游离出来。提取液经酸液中和后,高速离心去除杂质,取上清液并用微孔滤膜过滤,稀释后用高效液相色谱-质谱联用仪进行测定和确证。结果待测物质在2~100 ng/mL范围内呈良好的线性关系(r2=0.9941),左旋肉碱在不同基质中的加标回收率为66.3%~110.5%,精密度为2.0%~8.8%。结论此法可满足日常进出口乳品中左旋肉碱含量的测定工作,同其他方法相比具有准确定性、操作简单、省时高效的特点。
目的：建立高效液相色譜串聯質譜法測定乳品中左鏇肉堿的含量。方法樣品中的左鏇肉堿用乙腈水(3：1, V：V)溶液提取,提取液經堿皂化後使溶液中結閤態的左鏇肉堿遊離齣來。提取液經痠液中和後,高速離心去除雜質,取上清液併用微孔濾膜過濾,稀釋後用高效液相色譜-質譜聯用儀進行測定和確證。結果待測物質在2~100 ng/mL範圍內呈良好的線性關繫(r2=0.9941),左鏇肉堿在不同基質中的加標迴收率為66.3%~110.5%,精密度為2.0%~8.8%。結論此法可滿足日常進齣口乳品中左鏇肉堿含量的測定工作,同其他方法相比具有準確定性、操作簡單、省時高效的特點。
목적：건립고효액상색보천련질보법측정유품중좌선육감적함량。방법양품중적좌선육감용을정수(3：1, V：V)용액제취,제취액경감조화후사용액중결합태적좌선육감유리출래。제취액경산액중화후,고속리심거제잡질,취상청액병용미공려막과려,희석후용고효액상색보-질보련용의진행측정화학증。결과대측물질재2~100 ng/mL범위내정량호적선성관계(r2=0.9941),좌선육감재불동기질중적가표회수솔위66.3%~110.5%,정밀도위2.0%~8.8%。결론차법가만족일상진출구유품중좌선육감함량적측정공작,동기타방법상비구유준학정성、조작간단、성시고효적특점。
ABSTRACT:Objective To establish a method for the detection ofL-carnitine in milk products by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS).MethodsL-carnitine in samples was extracted by acetonitrile:water (3:1, V:V) solution. The combinedL-carnitine was released by alkaline saponification. After neutralized by acid, the extraction was centrifuged to discard impurity. Then superna-tant was filtered by syringe filter (0.2μm) and diluted. Finally, the sample was measured by HPLC-MS/MS. ResultsIt showed a good linearity of material in 2~100 ng/mL (r2=0.9941). The recoveries ofL-carnitine in different matrix were 66.3%~110.5%, with the precision of 2.0%~8.8%.ConclusionThis method can meet the daily inspection work of import and export ofL-carnitine in milk products. Compared with other methods, it has the characteristics of exact determination, simple operation, time saving and high efficiency.
