CAJ | 학술논문

选择4种不同花色的矮牵牛品种‘Pink’、‘Red’、‘Pink Vein’和‘Peppermint’，以MS培养基为基础培养基，分别添加不同质量浓度的6－BA、NAA、IBA激素，除草剂双丙氨膦和抗生素壮观霉素，研究其不定芽的诱导频率，为后续的叶绿体转化提供受体。结果表明：影响矮牵牛叶片诱导不定芽因素中，品种间的影响比激素更重要。当诱导培养基中的6－BA质量浓度为1．0 mg·L－1、NAA质量浓度为0．5 mg·L－1时，诱导效果最佳，是品种‘Pink’、‘Pink Vein’的最适培养基；同样地，品种‘Peppermint’最适培养基中，6－BA质量浓度不变、NAA的质量浓度降低至0．3 mg·L－1；品种‘Red’最适培养基中，6－BA质量浓度仍不变、NAA质量浓度降低至0．1 mg·L－1。4个矮牵牛品种的不定芽诱导率，由高到低的顺序为‘Pink’、‘Pink Vein’、‘Peppermint’、‘Red’，因此，优先选择品种‘Pink’作为转基因受体材料。分别利用不同质量浓度双丙氨膦和壮观霉素筛选，通过二者对品种‘Pink’叶片分化的影响，发现双丙氨膦和壮观霉素的最低抑制质量浓度分别为3、100 mg·L－1。该研究结果对今后利用叶绿体遗传转化技术改良矮牵牛品种提供了技术支持。
선택4충불동화색적왜견우품충‘Pink’、‘Red’、‘Pink Vein’화‘Peppermint’，이MS배양기위기출배양기，분별첨가불동질량농도적6－BA、NAA、IBA격소，제초제쌍병안련화항생소장관매소，연구기불정아적유도빈솔，위후속적협록체전화제공수체。결과표명：영향왜견우협편유도불정아인소중，품충간적영향비격소경중요。당유도배양기중적6－BA질량농도위1．0 mg·L－1、NAA질량농도위0．5 mg·L－1시，유도효과최가，시품충‘Pink’、‘Pink Vein’적최괄배양기；동양지，품충‘Peppermint’최괄배양기중，6－BA질량농도불변、NAA적질량농도강저지0．3 mg·L－1；품충‘Red’최괄배양기중，6－BA질량농도잉불변、NAA질량농도강저지0．1 mg·L－1。4개왜견우품충적불정아유도솔，유고도저적순서위‘Pink’、‘Pink Vein’、‘Peppermint’、‘Red’，인차，우선선택품충‘Pink’작위전기인수체재료。분별이용불동질량농도쌍병안련화장관매소사선，통과이자대품충‘Pink’협편분화적영향，발현쌍병안련화장관매소적최저억제질량농도분별위3、100 mg·L－1。해연구결과대금후이용협록체유전전화기술개량왜견우품충제공료기술지지。
With four Petunia hybrids cultivars with different color involving‘Pink’ ,‘Red’ ,‘Pink Vein’ and‘Peppermint’ , we studied the frequency of adventitious bud induction and then subsequently provided explants for chloroplast transforma?tion. The MS medium was served as basic medium supplemented with different concentrations of 6-BA, NAA and IBA, re?spectively, with the addition of bialaphos sodium and spectinomycin. The impact of cultivar on induction of adventitious buds from leaves of Petunia hybrid is more visible than that of hormone. The induction frequency of adventitious buds will be highest when the media were added with concentration of 1.0 mg·L-1 6-BA and 0.5 mg·L-1 NAA for ‘Pink’ and‘Pink Vein’. Similarly, the concentration of NAA will be lower to 0.3 mg·L-1 for ‘Peppermint’ and 0.1 mg·L-1 for‘Red’ , while the concentration of 6-BA keep the same as above in the optimal medium. According to the order from high to low, the ranking of frequencies of adventitious bud induction for four cultivars is‘Pink’ ,‘Pink Vein’ ,‘Peppermint’ and‘Red’ .‘Pink’ , therefore, is considered to be the suitable explant for the genetic transformation. The minimum inhib?itory concentration of bialaphos?sodium and spectinomycin in induction medium for ‘Pink’ is 3 mg·L-1 and 100 mg· L-1 , respectively. Our results will provide technical support for chloroplast transformation of Petunia hybrid to implement genetic improvement.