CAJ | 학술논문

目的：阐明黄精“九蒸九制”过程中其多糖和皂苷变化的科学内涵。方法：以葡萄糖和薯蓣皂苷元为指标成分，采用紫外－可见分光光度法，测定黄精“九蒸九制”过程中多糖和皂苷的含量变化，并通过单因素和正交试验，优化蒸制黄精中皂苷的超声辅助提取工艺，结果：随着蒸制次数的增加，多糖含量先减少后趋于稳定，皂苷先增加后趋于稳定。超声辅助提取皂苷的最佳工艺：75%乙醇，超声时间40 min，浸提温度70℃，提取一次。结论：优选出的炮制工艺重复性好，能有效提高皂苷的提取效率，具有良好的应用前景。
목적：천명황정“구증구제”과정중기다당화조감변화적과학내함。방법：이포도당화서여조감원위지표성분，채용자외－가견분광광도법，측정황정“구증구제”과정중다당화조감적함량변화，병통과단인소화정교시험，우화증제황정중조감적초성보조제취공예，결과：수착증제차수적증가，다당함량선감소후추우은정，조감선증가후추우은정。초성보조제취조감적최가공예：75%을순，초성시간40 min，침제온도70℃，제취일차。결론：우선출적포제공예중복성호，능유효제고조감적제취효솔，구유량호적응용전경。
ObjectiveTo explore the scientific basis of processing technology of Polygonatum sibiricum Red. By the change of polysaccharides and saponins constituents during nine-steam-nine-bask processing.MethodsThe polysaccharides and saponins constituents determined with the markers of glucose and diosgenin by Uv-vis spectrophotometry. The ultrasonic ex-traction technology for saponins was optimized by a single factor experiment and an orthogonal experiment.ResultsThe content of polysaccharides will decrease with the streaming times increasing and saponins increased before they were the stable. The optimal extract conditions were obtained as folows: ethanol concentration 75%, ultrasonic time 40 min, the extraction tempera-ture 70℃, only one time.ConclusionThe optimal extraction method was repeatable. It can significantly increases the yield of saponins, and has a promising prospect of practical applications.