CAJ | 학술논문

目的：探讨细胞外基质金属蛋白酶诱导因子在 SH-SY5Y 细胞中的表达及其与泛素表达的定位关系。方法：采用不同浓度的 MG-132蛋白酶体抑制剂（0、0.5、1、2.5和5μmol/L）处理体外培养人神经母细胞瘤细胞株 SH-SY5Y 细胞。处理24 h 后，采用 In-cell Western 法和 Western Blot 法检测 SH-SY5Y 细胞中 CD147蛋白的表达，采用免疫荧光双标法检测 CD147和泛素的共表达。结果：不同浓度的 MG-132蛋白酶体抑制剂处理 SH-SY5Y 细胞，CD147蛋白的含量呈浓度依赖性增加。当抑制剂浓度为5μmol/L 时，CD147的含量明显高于抑制剂浓度为0μmol/L 组，差异有统计学意义（P＜0.05）。SH-SY5Y 细胞中同时存在 CD147和泛素的表达，而且存在共表达部位。结论：CD147可能通过泛素-蛋白酶体途径降解。
목적：탐토세포외기질금속단백매유도인자재 SH-SY5Y 세포중적표체급기여범소표체적정위관계。방법：채용불동농도적 MG-132단백매체억제제（0、0.5、1、2.5화5μmol/L）처리체외배양인신경모세포류세포주 SH-SY5Y 세포。처리24 h 후，채용 In-cell Western 법화 Western Blot 법검측 SH-SY5Y 세포중 CD147단백적표체，채용면역형광쌍표법검측 CD147화범소적공표체。결과：불동농도적 MG-132단백매체억제제처리 SH-SY5Y 세포，CD147단백적함량정농도의뢰성증가。당억제제농도위5μmol/L 시，CD147적함량명현고우억제제농도위0μmol/L 조，차이유통계학의의（P＜0.05）。SH-SY5Y 세포중동시존재 CD147화범소적표체，이차존재공표체부위。결론：CD147가능통과범소-단백매체도경강해。
Objective: To investigate the expression of extracellular matrix metalloproteinase inducer in SH-SY5Y cells and the spacial relationship with the expression of ubiquitin. Methods: Human neuroblastoma cell line SH-SY5Y was treated with MG-132 proteasome inhibitors at different concentrations (0, 0.5, 1, 2.5 and 5 μmol/L) in vitro. After 24 h, In-cell Western and Western Blot methods were employed to detect the expression of CD147 protein. Immunofluorescence double labeling method was used to detect the co-localization of ubiquitin and CD147. Results:When MG-132 proteasome inhibitors were used to deal with SH-SY5Y cultured in vitro, The ex-pression of CD147 protein in SH-SY5Y cells was increased with MG-132 proteasome inhibitors in a concentration dependent manner. When the inhibitor concentration was 5 μmol/L, the expression of CD147 was significantly higher than that in the control (0 μmol/L) (P<0.05). The expressions of Ubiquitin and CD147 were co-localized in SH-SY5Y cells. Conclusion: CD147 may be degraded by the ubiquitin-proteasome pathway.