广西植物
廣西植物
엄서식물
Guihaia
2015年
6期
930-934
,共5页
沈薇%石晓峰%宁红霞%胡彩香
瀋薇%石曉峰%寧紅霞%鬍綵香
침미%석효봉%저홍하%호채향
雪松%原花青素%含量测定%紫外-可见分光光度法%DPPH 自由基
雪鬆%原花青素%含量測定%紫外-可見分光光度法%DPPH 自由基
설송%원화청소%함량측정%자외-가견분광광도법%DPPH 자유기
Cedrus deodara%proanthocyanidin%content determination%ultraviolet-visible spectrophotometer%DPPH free radical
雪松(Cedrus deodara )是松科(Pianaceae)雪松属(Cedrus )树种的泛称，为广泛分布于喜马拉雅山脉的常绿树种，包括雪松(C ．deodara )、黎巴嫩雪松(C ．libani )、短叶雪松(C ．brevifolia )和北非雪松(C ．atlan-tica )等。雪松的针叶药用历史悠久，主要化学成分为黄酮类、苯丙素类、有机酸类、三萜类、甾体类、多糖及针叶胶等，具有抗肿瘤、抗氧化、改善记忆、抗菌及抗病毒等多种功效。为了测定雪松不同部位中原花青素的含量，并考察其清除 DPPH 自由基活性的能力，该研究以 Fe3＋离子为催化剂，用正丁醇－盐酸法分别测定雪松松针、花序、小枝、木质部、树皮中原花青素的含量，并对不同部位提取液清除 DPPH 自由基的能力进行测定。结果表明：松针、花序、木质部、树皮、小枝中原花青素的含量分别为12．93％、12．93％、6．41％、12．00％、3．37％，在质量浓度(以生药计)为0．8 mg.mL-1时，其对 DPPH 自由基的清除率均达到或接近90％。该研究结果提示雪松各个部位的原花青素含量丰富，其提取液的体外抗氧化活性很高，这为雪松的进一步开发利用提供了很好的参考价值。
雪鬆(Cedrus deodara )是鬆科(Pianaceae)雪鬆屬(Cedrus )樹種的汎稱，為廣汎分佈于喜馬拉雅山脈的常綠樹種，包括雪鬆(C ．deodara )、黎巴嫩雪鬆(C ．libani )、短葉雪鬆(C ．brevifolia )和北非雪鬆(C ．atlan-tica )等。雪鬆的針葉藥用歷史悠久，主要化學成分為黃酮類、苯丙素類、有機痠類、三萜類、甾體類、多糖及針葉膠等，具有抗腫瘤、抗氧化、改善記憶、抗菌及抗病毒等多種功效。為瞭測定雪鬆不同部位中原花青素的含量，併攷察其清除 DPPH 自由基活性的能力，該研究以 Fe3＋離子為催化劑，用正丁醇－鹽痠法分彆測定雪鬆鬆針、花序、小枝、木質部、樹皮中原花青素的含量，併對不同部位提取液清除 DPPH 自由基的能力進行測定。結果錶明：鬆針、花序、木質部、樹皮、小枝中原花青素的含量分彆為12．93％、12．93％、6．41％、12．00％、3．37％，在質量濃度(以生藥計)為0．8 mg.mL-1時，其對 DPPH 自由基的清除率均達到或接近90％。該研究結果提示雪鬆各箇部位的原花青素含量豐富，其提取液的體外抗氧化活性很高，這為雪鬆的進一步開髮利用提供瞭很好的參攷價值。
설송(Cedrus deodara )시송과(Pianaceae)설송속(Cedrus )수충적범칭，위엄범분포우희마랍아산맥적상록수충，포괄설송(C ．deodara )、려파눈설송(C ．libani )、단협설송(C ．brevifolia )화북비설송(C ．atlan-tica )등。설송적침협약용역사유구，주요화학성분위황동류、분병소류、유궤산류、삼첩류、치체류、다당급침협효등，구유항종류、항양화、개선기억、항균급항병독등다충공효。위료측정설송불동부위중원화청소적함량，병고찰기청제 DPPH 자유기활성적능력，해연구이 Fe3＋리자위최화제，용정정순－염산법분별측정설송송침、화서、소지、목질부、수피중원화청소적함량，병대불동부위제취액청제 DPPH 자유기적능력진행측정。결과표명：송침、화서、목질부、수피、소지중원화청소적함량분별위12．93％、12．93％、6．41％、12．00％、3．37％，재질량농도(이생약계)위0．8 mg.mL-1시，기대 DPPH 자유기적청제솔균체도혹접근90％。해연구결과제시설송각개부위적원화청소함량봉부，기제취액적체외항양화활성흔고，저위설송적진일보개발이용제공료흔호적삼고개치。
Cedrus deodara (Pinaceae family),which consists of C .deodara ,C .libani ,C .brevifolia and C .atlan-tica ,is an evergreen tree growing extensively on the slopes of the Himalayas.Its leaves was a kind of chinese medi-cine for a long history.Its main chemical constituents include flavonoids,phenylpropanoids,organicacid,triterpenes, steroids,polysaccharides leaf glue and so on.The major pharmacological activities of pine needles of C .deodara in-clude antitumor,antioxidation,improving memory,antibacterial and antivirus,ect.In order to determine proantho-cyanidin content and DPPH radical scavenging activity of different parts from C .deodara .In this study,Fe3+ ion was used for catalysator,n-BuOH∶HCl (95∶5)was chromogenic agent and the detection wave-lengths were at 550 nm. And the content of proanthocyanidin was determined in different parts from C .deodara by ultraviolet-visible spectro-photometer.And antioxidant activity of extract from different parts of C .deodara were investigated by scavenging 1,1-Diphenyl-2-picrylhydrazyl free radical (DPPH).The results showed that proanthocyanidins content in different parts of C .deodara was pine needles(12.93%),inflorescence (12.93%),bark(12.00%),wood (6.41%),tender branchlets(3.37%).The extract from different parts of C .deodara had good antioxidant activity.In a certain concen-tration scope,there was a strong positive correlation between the scavenging activity and the concentrations.The or-der of IC50 was tender branchlets > pine needles > wood> bark > inflorescence.Except for branchlets,their DPPH scavenging activities were around 90% while the concentration of the extract from different parts was 0.8 mg.mL-1 . The results indicated that there were higher proanthocyanidins content and antioxidant activities in different parts of C .deodara ,which provide the reference on comprehensive development and utilization of C .deodara .
