海洋渔业
海洋漁業
해양어업
Marine Fisheries
2015年
6期
533-540
,共8页
王建建%施兆鸿%高权新%彭士明
王建建%施兆鴻%高權新%彭士明
왕건건%시조홍%고권신%팽사명
银鲳%产酶菌%温度%pH%发酵时间
銀鯧%產酶菌%溫度%pH%髮酵時間
은창%산매균%온도%pH%발효시간
silver pomfret (Pampus argenteus)%enzyme-producing bacteria%temperature%pH%fermentation time
采用酶学分析法研究了单因子（温度、pH、发酵时间）变化对野生银鲳（Pampus argenteus）消化道内同时分泌蛋白酶、淀粉酶、纤维素酶和脂肪酶中两种以上的5株菌株产酶活力的影响，即通过测定酶活力的大小找到每株菌最适产酶条件范围。结果显示，在温度31～37℃、pH 7～8、发酵时间3 d的条件下，5株产酶菌的蛋白酶活力最大；在不同温度和pH条件下，5菌株分泌的淀粉酶活力各不相同，但都随发酵时间的延长而增大，3～5 d达最大值；4株分泌纤维素酶的菌株纤维素酶活力受环境影响总体变化不大，在温度31～37℃、酸性条件（pH 5）和发酵时间4 d的条件下为活力最大；2株分泌脂肪酶的菌株在28～31℃、中性（pH 7）、发酵时间1 d条件下其脂肪酶活力最大，最大可接近180 U·mL－1，但活力都随发酵时间而显著下降。结果表明，不同产酶菌所分泌的消化酶活力所需的最适条件有很大不同，了解和掌握银鲳肠道中菌群的产酶条件，对开发潜在产酶益生菌有着积极的作用。
採用酶學分析法研究瞭單因子（溫度、pH、髮酵時間）變化對野生銀鯧（Pampus argenteus）消化道內同時分泌蛋白酶、澱粉酶、纖維素酶和脂肪酶中兩種以上的5株菌株產酶活力的影響，即通過測定酶活力的大小找到每株菌最適產酶條件範圍。結果顯示，在溫度31～37℃、pH 7～8、髮酵時間3 d的條件下，5株產酶菌的蛋白酶活力最大；在不同溫度和pH條件下，5菌株分泌的澱粉酶活力各不相同，但都隨髮酵時間的延長而增大，3～5 d達最大值；4株分泌纖維素酶的菌株纖維素酶活力受環境影響總體變化不大，在溫度31～37℃、痠性條件（pH 5）和髮酵時間4 d的條件下為活力最大；2株分泌脂肪酶的菌株在28～31℃、中性（pH 7）、髮酵時間1 d條件下其脂肪酶活力最大，最大可接近180 U·mL－1，但活力都隨髮酵時間而顯著下降。結果錶明，不同產酶菌所分泌的消化酶活力所需的最適條件有很大不同，瞭解和掌握銀鯧腸道中菌群的產酶條件，對開髮潛在產酶益生菌有著積極的作用。
채용매학분석법연구료단인자（온도、pH、발효시간）변화대야생은창（Pampus argenteus）소화도내동시분비단백매、정분매、섬유소매화지방매중량충이상적5주균주산매활력적영향，즉통과측정매활력적대소조도매주균최괄산매조건범위。결과현시，재온도31～37℃、pH 7～8、발효시간3 d적조건하，5주산매균적단백매활력최대；재불동온도화pH조건하，5균주분비적정분매활력각불상동，단도수발효시간적연장이증대，3～5 d체최대치；4주분비섬유소매적균주섬유소매활력수배경영향총체변화불대，재온도31～37℃、산성조건（pH 5）화발효시간4 d적조건하위활력최대；2주분비지방매적균주재28～31℃、중성（pH 7）、발효시간1 d조건하기지방매활력최대，최대가접근180 U·mL－1，단활력도수발효시간이현저하강。결과표명，불동산매균소분비적소화매활력소수적최괄조건유흔대불동，료해화장악은창장도중균군적산매조건，대개발잠재산매익생균유착적겁적작용。
In this study,the effects of single factor variation (temperature,pH and fermentation time)on activities of two or more enzymes,including protease,amylase,cellulose and lipase produced simultaneously by enzyme-producing bacteria in the digestive tract of wild silver pomfret (Pampus argenteus)were measured using the method of enzymatic analysis.The aim is to find the optimum range of fermentation conditions for each strain by measuring the enzyme activity by different media,different incubation temperatures,pH and fermentation time.Results showed that the activities of protease produced by five strains of enzyme-producing bacteria were the highest in the conditions of temperature 3 1 -37 ℃,pH 7 -8 and fermentation time 3 d. The activities of cellulose produced by four cellulose-producing bacteria were not affected significantly by environmental conditions;the enzyme activity was the lowest among all enzymes,and reached the maximum in the conditions of temperature 3 1 -37 ℃,pH 5 and fermentation time 4 d.The activities of lipase produced by four lipase-producing bacteria reached the maximum (180 U·mL-1 )in the conditions of temperature 28-31 ℃,pH 7 and fermentation time 1 d,and declined with the increasing fermentation time.These results indicated that the optimum conditions for enzyme-producing bacteria secreting enzymes with the highest activities were significantly different.Understanding and grasping the enzyme-producing conditions of bacteria in the gut of Pampus argenteus could help to exploite potential enzymes-producing probiotics.
