CAJ | 학술논문

蛋白磷酸酶(protein phosphatase,PP)是蛋白质可逆磷酸化调节机制中的关键酶,而 PP2C 磷酸酶是一类丝氨酸/苏氨酸残基蛋白磷酸酶,是高等植物中最大的蛋白磷酸酶家族,包含76个家族成员,广泛存在于生物体中。迄今为止,在植物体内已经发现了4种 PP2C 蛋白磷酸酶。蛋白激酶和蛋白磷酸酶协同催化蛋白质可逆磷酸化,在植物体内信号转导和生理代谢中起着重要的调节作用,蛋白质的磷酸化几乎存在于所有的信号转导途径中。大量研究表明,PP2Cs 参与多条信号转导途径,包括PP 2C 参与 ABA 调控,对干旱、低温、高盐等逆境胁迫的响应,参与植物创伤和种子休眠或萌发等信号途径,其调控机制不同,但酶催化活性都依赖于 Mg2＋或 Mn2＋的浓度。植物 PP2C 蛋白的 C 端催化结构域高度保守,而 N 端功能各异。文中还综述了高等植物PP 2C 的分类、结构、ABA 受体与 PP2Cs 蛋白互作、PP2C 基因参与 ABA 信号途径以及其他逆境信号转导途径的研究进展。
단백린산매(protein phosphatase,PP)시단백질가역린산화조절궤제중적관건매,이 PP2C 린산매시일류사안산/소안산잔기단백린산매,시고등식물중최대적단백린산매가족,포함76개가족성원,엄범존재우생물체중。흘금위지,재식물체내이경발현료4충 PP2C 단백린산매。단백격매화단백린산매협동최화단백질가역린산화,재식물체내신호전도화생리대사중기착중요적조절작용,단백질적린산화궤호존재우소유적신호전도도경중。대량연구표명,PP2Cs 삼여다조신호전도도경,포괄PP 2C 삼여 ABA 조공,대간한、저온、고염등역경협박적향응,삼여식물창상화충자휴면혹맹발등신호도경,기조공궤제불동,단매최화활성도의뢰우 Mg2＋혹 Mn2＋적농도。식물 PP2C 단백적 C 단최화결구역고도보수,이 N 단공능각이。문중환종술료고등식물PP 2C 적분류、결구、ABA 수체여 PP2Cs 단백호작、PP2C 기인삼여 ABA 신호도경이급기타역경신호전도도경적연구진전。
Protein phosphatase is the most important and pivotal enzymes in reversible protein phosphorylation regula-ting mechanisms.While the PP2C phosphatase is a kind of serine/threonine residues of protein phosphatase,is the largest protein phosphatase family in higher plants,there are 76 family members,widely exists in living organisms. So far,four kinds of PP2C protein phosphatases have been found in plants.Protein kinase and protein phosphatase catalyzed reversible protein phosphorylation,play an important role in plant signal transduction and physiological me-tabolism,protein phosphorylation exist in almost the signal transduction pathway.Numerous academic studies have shown that plant PP2Cs are involved in multiple signal transduction pathways including PP 2C involved in ABA sig-naling pathway,the response to drought,low temperature,salt stress,participated in the plant wound and seed dor-mancy or germination signal pathway,and exist the different regulation mechanism and the enzyme catalytic activity were dependent on the concentrations of Mg2+ or Mn2+ .In plant PP2Cs protein C-terminal,there are a highly con-served catalytic domains,as well as in their N-terminal,their function are different.The review would provide a brief overview of classification,structure of PP 2Cs ,the interaction between ABA receptor and PP2Cs protein,the recent progresses about their roles in ABA and other stress signal transduction pathway in higher plant.