CAJ | 학술논문

目的：探讨 Ghrelin 对小鼠认知功能的影响及机制。方法：野生型（WT）组和 CGRP 基因敲除（CGRP-/-）组小鼠各10只，且各分为对照亚组和实验亚组各5只，均腹腔注射100μL 含0.1μg/L Gherlin 的 PBS 试液（实验亚组）或空白 PBS 试液（对照亚组），进行 Morris 水迷宫实验及记忆探索实验，并测定其海马趾组织中CGRP、IGF-1及 IGF-1 mRNA 含量。结果：WT 组中，实验亚组与对照亚组比较，逃避潜伏期时间缩短，目标区域停留时间百分比增加，CGRP、IGF-1及 IGF-1 mRNA 的含量较高（P＜0.05）；CGRP-/-组中，实验亚组与对照亚组相比差异无统计学意义（P＞0.05）；与 CGRP-/-组实验亚组比较，WT 组实验亚组注射第4、5天的逃避潜伏期较低，目标区域停留时间百分比较高，CGRP、IGF-1及 IGF-1 mRNA 含量较高，差异有统计学意义（P＜0.05）。结论：Gherlin 可能通过促进海马体释放 CGRP，使得海马的 IGF-1产量增加，由此提高小鼠的认知能力。
목적：탐토 Ghrelin 대소서인지공능적영향급궤제。방법：야생형（WT）조화 CGRP 기인고제（CGRP-/-）조소서각10지，차각분위대조아조화실험아조각5지，균복강주사100μL 함0.1μg/L Gherlin 적 PBS 시액（실험아조）혹공백 PBS 시액（대조아조），진행 Morris 수미궁실험급기억탐색실험，병측정기해마지조직중CGRP、IGF-1급 IGF-1 mRNA 함량。결과：WT 조중，실험아조여대조아조비교，도피잠복기시간축단，목표구역정류시간백분비증가，CGRP、IGF-1급 IGF-1 mRNA 적함량교고（P＜0.05）；CGRP-/-조중，실험아조여대조아조상비차이무통계학의의（P＞0.05）；여 CGRP-/-조실험아조비교，WT 조실험아조주사제4、5천적도피잠복기교저，목표구역정류시간백분비교고，CGRP、IGF-1급 IGF-1 mRNA 함량교고，차이유통계학의의（P＜0.05）。결론：Gherlin 가능통과촉진해마체석방 CGRP，사득해마적 IGF-1산량증가，유차제고소서적인지능력。
Objective:To explore the effect and mechanism of Ghrelin on the cognitive function in mice. Methods:Ten wild-type mice (WT group) and 10 CGRP-gene knockout mice (CGRP-/- group)were equally divided as the control subgroup (n=5) and the examination subgroup (n=5). Mice were treated by intraperitoneal injection of 100μL of 0.1 μg/L Ghrelin solution dissolved in PBS (examination subgroup) or with PBS alone (control subgroup). The Morris maze task and probe test were examined in both groups, and then the tissue levels of CGRP, IGF-1 and IGF-1 mRNA in the hippocampus were determined. Results: In the WT group, the escape latency of the examina-tion subgroup was shortened, the time spent in the target area of the examination subgroup was significantly en-hanced, the tissue levels of CGRP, IGF-1 and IGF-1 mRNA were significantly up-regulated (P<0.05) compared to those in the control subgroup. There was no statistically significance between the two subgroups in CGRP-/- group (P>0.05). Compared with the examination subgroup of CGRP-/- group, the escape latency of the examination sub-group of WT group was shorter on days 4 and 5, and the time spent in the target area was longer, and the tissue lev-els of CGRP, IGF-1 and IGF-1 mRNA were significantly higher (P<0.05). Conclusion: Ghrelin may increase the hippocampal expression of IGF-1 via increase of CGRP release from the hippocampus, thus cognitive function was improved.