CAJ | 학술논문

目的：应用双向荧光差异凝胶电泳（2-D DIGE）和质谱技术筛选、鉴定与口腔扁平苔藓（OLP）患者唾液有关的差异蛋白。方法：收集3例 OLP 患者及3例健康人唾液，提取唾液总蛋白，采用2-D DIGE 技术分离蛋白质，寻找差异蛋白质点，应用液相色谱－质谱联用技术（LC-MS）对差异蛋白质点进行质谱鉴定。结果：SDS －聚丙烯酰胺凝胶电泳（SDS-PAGE）显示各样品条带清晰，无明显蛋白质丢失，经2-D DIGE 分离蛋白质后，蛋白质点重复性较好。OLP 患者组和健康对照组唾液差异蛋白质点数平均为（317±71）个，用质谱技术鉴定了重复性较好的4个差异蛋白质点（分泌型 IgA1、锌α-2-糖蛋白、唾液淀粉酶、血清白蛋白），在 OLP 患者唾液中表达量均上调。结论：分泌型 IgA1、锌α-2-糖蛋白、唾液淀粉酶、血清白蛋白在 OLP 患者唾液中高表达，可能参与了 OLP 的发生、发展。
목적：응용쌍향형광차이응효전영（2-D DIGE）화질보기술사선、감정여구강편평태선（OLP）환자타액유관적차이단백。방법：수집3례 OLP 환자급3례건강인타액，제취타액총단백，채용2-D DIGE 기술분리단백질，심조차이단백질점，응용액상색보－질보련용기술（LC-MS）대차이단백질점진행질보감정。결과：SDS －취병희선알응효전영（SDS-PAGE）현시각양품조대청석，무명현단백질주실，경2-D DIGE 분리단백질후，단백질점중복성교호。OLP 환자조화건강대조조타액차이단백질점수평균위（317±71）개，용질보기술감정료중복성교호적4개차이단백질점（분비형 IgA1、자α-2-당단백、타액정분매、혈청백단백），재 OLP 환자타액중표체량균상조。결론：분비형 IgA1、자α-2-당단백、타액정분매、혈청백단백재 OLP 환자타액중고표체，가능삼여료 OLP 적발생、발전。
Objective:To identify differentially expressed saliva proteins of oral lichen planus(OLP)patients by two-dimensional fluo-rescence difference electrophoresis(2-D DIGE)and mass spectrometry(MS).Methods:3 pairs of saliva samples from OLP patients and matched healthy adults were collected.Saliva proteins were separated by 2-D DIGE and identified by liquid chromatography-mass spectrometry(LC-MS).Results:SDS-PAGE examination showed that the electrophoresis bands were clear and protein loss was rare. Protein dots were highly reproducible by 2-D DIGE.In average,the abundance of (31 7 ±71 )saliva protein spots were found in OLP pa-tients.4 highly reproducible spots were identified to be secretory IgA1 ,zincα-2-glycoprotein,salivary amylase and serum albumin by LC-MS and they were at higher level in OLP patients than those in the healthy controls.Conclusion:Secretory IgA1 ,zincα-2-glyco-protein,salivary amylase and serum albumin are highly expressed in the saliva of OLP patients,and may be related to the occurrence and development of oral lichen planus.