胃肠病学
胃腸病學
위장병학
Chinese Journal of Gastroenterology
2015年
11期
672-675
,共4页
李清华%杨志文%吴梅%徐萍
李清華%楊誌文%吳梅%徐萍
리청화%양지문%오매%서평
结直肠腺瘤%复发%微RNAs%差异表达%微阵列分析
結直腸腺瘤%複髮%微RNAs%差異錶達%微陣列分析
결직장선류%복발%미RNAs%차이표체%미진렬분석
Colorectal Adenoma%Recurrence%MicroRNAs%Differential Expression%Microarray Analysis
背景：miRNAs是一类非编码小分子RNA，可在转录后水平调控基因表达，在肿瘤发生、发展中发挥重要作用。目的：以miRNA芯片筛选在结直肠腺瘤复发患者外周血中特异性表达的miRNAs，以期为复发预测提供敏感而特异的指标。方法：选取50例曾行结肠镜下结直肠腺瘤摘除术的患者，于2012年8月—2013年9月复查结肠镜，从中选取结直肠腺瘤复发者和未复发者各4例，采集外周血标本，以miRNA芯片检测miRNAs表达谱并筛选复发组与未复发组间的差异表达miRNAs。以real-time PCR对部分差异表达miRNAs进行验证。结果：与未复发组相比，复发组共筛选出11个差异表达miRNAs，其中7个表达上调，分别为hp hsa-miR-548ai、hsa-miR-4446-3p、hsa-miR-139-3p、hsa-miR-27a-5p、hsa-miR-10a、hsa-miR-23a、hsa-miR-486-3p，4个表达下调，分别为 hsa-miR-124、hsa-miR-3613-5p、hsa-miR-495、hsa-miR-485-5p。其中7个miRNAs经real-time PCR验证，表达变化趋势与芯片检测结果相一致。结论：部分差异表达miRNAs可能参与了结直肠腺瘤的复发机制，为进一步探索复发预测指标提供了线索。
揹景：miRNAs是一類非編碼小分子RNA，可在轉錄後水平調控基因錶達，在腫瘤髮生、髮展中髮揮重要作用。目的：以miRNA芯片篩選在結直腸腺瘤複髮患者外週血中特異性錶達的miRNAs，以期為複髮預測提供敏感而特異的指標。方法：選取50例曾行結腸鏡下結直腸腺瘤摘除術的患者，于2012年8月—2013年9月複查結腸鏡，從中選取結直腸腺瘤複髮者和未複髮者各4例，採集外週血標本，以miRNA芯片檢測miRNAs錶達譜併篩選複髮組與未複髮組間的差異錶達miRNAs。以real-time PCR對部分差異錶達miRNAs進行驗證。結果：與未複髮組相比，複髮組共篩選齣11箇差異錶達miRNAs，其中7箇錶達上調，分彆為hp hsa-miR-548ai、hsa-miR-4446-3p、hsa-miR-139-3p、hsa-miR-27a-5p、hsa-miR-10a、hsa-miR-23a、hsa-miR-486-3p，4箇錶達下調，分彆為 hsa-miR-124、hsa-miR-3613-5p、hsa-miR-495、hsa-miR-485-5p。其中7箇miRNAs經real-time PCR驗證，錶達變化趨勢與芯片檢測結果相一緻。結論：部分差異錶達miRNAs可能參與瞭結直腸腺瘤的複髮機製，為進一步探索複髮預測指標提供瞭線索。
배경：miRNAs시일류비편마소분자RNA，가재전록후수평조공기인표체，재종류발생、발전중발휘중요작용。목적：이miRNA심편사선재결직장선류복발환자외주혈중특이성표체적miRNAs，이기위복발예측제공민감이특이적지표。방법：선취50례증행결장경하결직장선류적제술적환자，우2012년8월—2013년9월복사결장경，종중선취결직장선류복발자화미복발자각4례，채집외주혈표본，이miRNA심편검측miRNAs표체보병사선복발조여미복발조간적차이표체miRNAs。이real-time PCR대부분차이표체miRNAs진행험증。결과：여미복발조상비，복발조공사선출11개차이표체miRNAs，기중7개표체상조，분별위hp hsa-miR-548ai、hsa-miR-4446-3p、hsa-miR-139-3p、hsa-miR-27a-5p、hsa-miR-10a、hsa-miR-23a、hsa-miR-486-3p，4개표체하조，분별위 hsa-miR-124、hsa-miR-3613-5p、hsa-miR-495、hsa-miR-485-5p。기중7개miRNAs경real-time PCR험증，표체변화추세여심편검측결과상일치。결론：부분차이표체miRNAs가능삼여료결직장선류적복발궤제，위진일보탐색복발예측지표제공료선색。
Background:MicroRNAs ( miRNAs ) is a class of small non-coding RNAs that regulate gene expression at posttranscriptional level. They are considered to play a critical role in the tumorigenesis and development of various tumors. Aims:To identify the recurrence-specific miRNAs in peripheral blood of patients with recurrent colorectal adenoma by miRNA array for providing sensitive and specific molecular markers for recurrence prediction. Methods:Fifty colorectal adenoma patients who had undergone polypectomy by colonoscopy were re-examined from Aug. 2012 to Sep. 2013. Four recurrent and four non-recurrent patients were selected and miRNA array was used to detect the expression profiles of miRNAs in peripheral blood samples. Differentially expressed miRNAs were identified between recurrent and non-recurrent patients. Real-time PCR was applied to verify the differentially expressed miRNAs identified by microarray. Results:Seven up-regulatedmiRNAs(hp hsa-miR-548ai,hsa-miR-4446-3p,hsa-miR-139-3p,hsa-miR-27a-5p,hsa-miR-10a,hsa-miR-23a and hsa-miR-486-3p)and 4 down-regulated miRNAs( hsa-miR-124,hsa-miR-3613-5p,hsa-miR-495 and hsa-miR-485-5p)were identified by microarray between recurrent and non-recurrent patients. Among them,seven miRNAs were validated by real-time PCR as consistent with microarray. Conclusions:Some differentially expressed miRNAs may be involved in the recurrence of colorectal adenoma,which gives clues for further investigation on recurrence predictors.
