CAJ | 학술논문

目的：检测成牙本质细胞相关功能性蛋白（DSPP、DMP-1、ALP）在 BMP-7诱导前后的牙髓干细胞（dental pulp stem cells，DPSCs）中的表达。方法：采用电镜观察、CCK8和细胞免疫化学染色的方法，观察经100 ng／ml BMP-7诱导后的 DPSCs细胞形态、增殖和向成牙本质细胞方向分化的情况。结果：经 BMP-7诱导的 DPSCs 形态无明显变化；BMP-7诱导后第7天细胞增殖比对照组减慢。DPSCs 不表达或低表达 DSPP、DMP-1和 ALP 经 BMP-7诱导后，DSPP、DMP-1和 ALP 呈阳性表达。结论：DPSCs 经 BMP-7诱导后可以向成牙本质细胞方向分化。
목적：검측성아본질세포상관공능성단백（DSPP、DMP-1、ALP）재 BMP-7유도전후적아수간세포（dental pulp stem cells，DPSCs）중적표체。방법：채용전경관찰、CCK8화세포면역화학염색적방법，관찰경100 ng／ml BMP-7유도후적 DPSCs세포형태、증식화향성아본질세포방향분화적정황。결과：경 BMP-7유도적 DPSCs 형태무명현변화；BMP-7유도후제7천세포증식비대조조감만。DPSCs 불표체혹저표체 DSPP、DMP-1화 ALP 경 BMP-7유도후，DSPP、DMP-1화 ALP 정양성표체。결론：DPSCs 경 BMP-7유도후가이향성아본질세포방향분화。
Objective:To examine the expression of odontoblast related proteins in dental pulp stem cells(DPSCs)induced by BMP-7.Methods:DPSCs were cultured in the common culture medium or medium supplemented with 1 00 ng/ml BMP-7.Electron microscope,CCK8 and immunohistochemical staining were carried out to estimate the cell morphology and differentiation.Results:In-duced by BMP-7,the morphology of DPSCs was not changed,the proliferation of DPSCs was slower than that of the cells without BMP-7 treatment.DPSCs were negative for the expression of DSPP,DMP-7 and ALP.However,DPSCs were found strongly positive for DSPP,DMP-7 and ALP after the induction of BMP-7.Conclusion:BMP-7 induction may promote the differentiation of DPSCs.