口腔医学
口腔醫學
구강의학
Stomatology
2015年
11期
897-900
,共4页
王丽梅%刘字珍%吕再玲%蓝海
王麗梅%劉字珍%呂再玲%藍海
왕려매%류자진%려재령%람해
隔山消%釉质%再矿化%显微硬度%扫描电镜
隔山消%釉質%再礦化%顯微硬度%掃描電鏡
격산소%유질%재광화%현미경도%소묘전경
Geranium Strictipes R .Kunth%enamel%remineralization%microhardness%SEM
目的：通过测试隔山消不同溶剂提取物处理人工龋齿后的显微硬度，并用SEM观察其表面形态，研究隔山消不同溶剂提取物对人工脱矿釉质再矿化的影响。方法用化学方法建立人工脱矿釉质龋模型，以乙酸乙酯提取物，正丁醇提取物，水层提取物为实验溶液，20 mg／L的氟化钠为阳性对照，去离子水为阴性对照将其分为5组，经体外pH循环后，测试其显微硬度值，SEM观察表面形态。结果显微硬度显示隔山消3种不同溶剂提取物和NaF经再矿化处理后，各组釉质硬度较再矿化前明显增高（P＜0．05），去离子水组再矿化前后无明显差异（P＞0．05），各组组间比较有统计学意义（P＜0．05）。说明隔山消不同溶剂提取物和NaF对脱矿牛切牙有再矿化作用。 SEM显示，氟化钠组有紧密均匀的沉淀区，去离子水组表面呈疏松结构，正丁醇组有紧密的矿物沉积，并有很小的空隙，乙酸乙酯组矿物沉淀较少，并有较大空隙，水层组有规则的颗粒沉积物生成。结论隔山消不同溶剂提取物对人工脱矿釉质均有再矿化作用，其作用从强到弱为：水层提取部位，正丁醇提取部位，乙酸乙酯提取部位。
目的：通過測試隔山消不同溶劑提取物處理人工齲齒後的顯微硬度，併用SEM觀察其錶麵形態，研究隔山消不同溶劑提取物對人工脫礦釉質再礦化的影響。方法用化學方法建立人工脫礦釉質齲模型，以乙痠乙酯提取物，正丁醇提取物，水層提取物為實驗溶液，20 mg／L的氟化鈉為暘性對照，去離子水為陰性對照將其分為5組，經體外pH循環後，測試其顯微硬度值，SEM觀察錶麵形態。結果顯微硬度顯示隔山消3種不同溶劑提取物和NaF經再礦化處理後，各組釉質硬度較再礦化前明顯增高（P＜0．05），去離子水組再礦化前後無明顯差異（P＞0．05），各組組間比較有統計學意義（P＜0．05）。說明隔山消不同溶劑提取物和NaF對脫礦牛切牙有再礦化作用。 SEM顯示，氟化鈉組有緊密均勻的沉澱區，去離子水組錶麵呈疏鬆結構，正丁醇組有緊密的礦物沉積，併有很小的空隙，乙痠乙酯組礦物沉澱較少，併有較大空隙，水層組有規則的顆粒沉積物生成。結論隔山消不同溶劑提取物對人工脫礦釉質均有再礦化作用，其作用從彊到弱為：水層提取部位，正丁醇提取部位，乙痠乙酯提取部位。
목적：통과측시격산소불동용제제취물처리인공우치후적현미경도，병용SEM관찰기표면형태，연구격산소불동용제제취물대인공탈광유질재광화적영향。방법용화학방법건립인공탈광유질우모형，이을산을지제취물，정정순제취물，수층제취물위실험용액，20 mg／L적불화납위양성대조，거리자수위음성대조장기분위5조，경체외pH순배후，측시기현미경도치，SEM관찰표면형태。결과현미경도현시격산소3충불동용제제취물화NaF경재광화처리후，각조유질경도교재광화전명현증고（P＜0．05），거리자수조재광화전후무명현차이（P＞0．05），각조조간비교유통계학의의（P＜0．05）。설명격산소불동용제제취물화NaF대탈광우절아유재광화작용。 SEM현시，불화납조유긴밀균균적침정구，거리자수조표면정소송결구，정정순조유긴밀적광물침적，병유흔소적공극，을산을지조광물침정교소，병유교대공극，수층조유규칙적과립침적물생성。결론격산소불동용제제취물대인공탈광유질균유재광화작용，기작용종강도약위：수층제취부위，정정순제취부위，을산을지제취부위。
Objective To test microhardness of artificial caries which was treated by different extracts of Geranium Strictipes R . Kunth,to observe its surface morphology by SEM ,and to study the effects of different solvent extracts on remineralization of artificial demineralized enamel .Methods 5 groups of artificial demineralized enamel caries models were established by chemical methods :the group of ethyl acetate extract ,the group of butanol extract ,the group of water layer extract ,20 mg/mL NaF positive control group , and deionized water negative control group .After in vitro pH cycling ,microhardness was tested and the surface morphology was observed by SEM.Results Microhardness results showed that after the remineralization treatment by three different extracts and NaF ,the en-amel hardness in each group significantly increased than that before remineralization (P<0.05),while the deionized water group showed no significant remineralization difference (P>0.05),and comparison between groups was statistically significant (P<0.05), denoting the remineralization effects of different extracts of Geranium Strictipes R .Kunth and NaF on demineralized bovine incisors . SEM results showed there was uniform and close precipitation zone in NaF group ,loose surface structure in deionized water group ,close mineral deposits and a small gap in the group of butanol extract ,less mineral deposits and a larger gap in the group of ethyl acetate ex-tract,and regular particle deposits in the group of water layer extract .Conclusions The different extracts of Geranium Strictipes R . Kunth have remineralization effects on artificial demineralized enamel .The best effect was obtained by water layer extract ,then butanol extract,and lastly ethyl acetate extract .
