CAJ | 학술논문

采用不同浓度葡萄糖对间充质干细胞(MSCs)增殖的影响来研究运动员补糖的 MSCs效应，分别用5.0和22.5 mmol/L葡萄糖预处理小鼠MSCs细胞株C3H10T1/248 h后，换不同浓度葡萄糖(5.0~500.0 mmol/L）持续培养或50.0~150.0 mmol/L和22.5 mmol/L进行12 h交替培养，甲基噻唑基四唑(MTT)法检测24~192 h的细胞增殖活性。结果发现：1)22.5和50.0 mmol/L葡萄糖组细胞的增殖活性最佳，并称为正糖。浓度低于或高于正糖的葡萄糖称为低糖或高糖。2)22.5 mmol/L正糖预处理后换不同糖浓度持续培养时，和正糖相比，高糖100.0~300.0 mmol/L具有短暂促增殖作用。3)高糖长期培养抑制细胞增殖。结果说明：高糖对正糖预处理的MSCs具有短暂促增殖效应。
채용불동농도포도당대간충질간세포(MSCs)증식적영향래연구운동원보당적 MSCs효응，분별용5.0화22.5 mmol/L포도당예처리소서MSCs세포주C3H10T1/248 h후，환불동농도포도당(5.0~500.0 mmol/L）지속배양혹50.0~150.0 mmol/L화22.5 mmol/L진행12 h교체배양，갑기새서기사서(MTT)법검측24~192 h적세포증식활성。결과발현：1)22.5화50.0 mmol/L포도당조세포적증식활성최가，병칭위정당。농도저우혹고우정당적포도당칭위저당혹고당。2)22.5 mmol/L정당예처리후환불동당농도지속배양시，화정당상비，고당100.0~300.0 mmol/L구유단잠촉증식작용。3)고당장기배양억제세포증식。결과설명：고당대정당예처리적MSCs구유단잠촉증식효응。
In order to study the mesenchymal stem cells (MSCs) effect of carbohydrate supplementation for athletes by studying the effect of glucose in different concentrations on the proliferation of MSCs, the authors pretreated cell lines C3H10T1/2 of MSCs of mice with 5.0 and 22.5 mmol/L glucose respectively for 48 h, then continuously cul-tured them with glucose in different concentrations (5.0~500.0 mmol/L) or alternatively cultured them with 50.0~150.0 mmol/L and 22.5 mmol/L glucose for 12 h, measured the proliferation activity of 24~192 h cells by means of methylthiazolyldiphenyl-tetrazolium bromide/thiazolyl blue tetrazolium bromide (MTT) assay, and re-vealed the following findings: 1)the proliferation activity of the cells cultured with 22.5 and 50.0 mmol/L glucose was the best, and the glucose was called as normal glucose, while the glucose whose concentration was lower or higher than normal glucose was called as low glucose or high glucose; 2)when MSCs were pretreated with 22.5 mmol/L normal glucose and then continuously cultured with glucose in different concentrations, as compared with normal glucose, 100.0~300.0 mmol/L high glucose had a short-term proliferation promoting function; 3)longer-term high glucose culturing restrained cell proliferation. The said findings indicate that high glucose has a short-term pro-liferation promoting effect on MSCs pretreated with normal glucose.