CAJ | 학술논문

目的：通过动物实验研究镁硒制剂对小鼠氟牙症的影响，为氟牙症的防治提供基础数据。方法80只雄性SPF级ICR小鼠，随机分为对照组、单镁组、单硒组、镁硒组、加氟组、镁氟组、硒氟组、镁硒氟组八组。前四组饮用双蒸水，后四组饮用F－浓度为50 m g／L的双蒸水。对照组和加氟组常规饲料喂养，单镁组和镁氟组用添加MgSO4·7H2O 162．5 mg／kg的常规饲料喂养，单硒组和硒氟组用添加Na2SeO3·5H2O 2 mg／kg的常规饲料喂养，镁硒组和镁硒氟组用添加MgSO4·7 H2O 162．5 mg／kg＋ Na2SeO35· H2O 2 mg／kg的常规饲料喂养。饲养42 d ，第42天时观察氟牙症的发生情况，并处死动物获取切牙标本，H E染色观察成釉细胞形态。结果加氟组小鼠切牙成釉细胞扭曲变形，细胞内出现空泡，Tomes突消失，氟牙症发病率为100％；镁硒氟组小鼠成釉细胞形态较加氟组明显改善，氟牙症发病率为40％，与加氟组差异明显（ P＜0．01）。结论氟对小鼠切牙成釉细胞有毒性作用，造成氟牙症，镁硒可拮抗其作用，减轻氟牙症的病变程度。
목적：통과동물실험연구미서제제대소서불아증적영향，위불아증적방치제공기출수거。방법80지웅성SPF급ICR소서，수궤분위대조조、단미조、단서조、미서조、가불조、미불조、서불조、미서불조팔조。전사조음용쌍증수，후사조음용F－농도위50 m g／L적쌍증수。대조조화가불조상규사료위양，단미조화미불조용첨가MgSO4·7H2O 162．5 mg／kg적상규사료위양，단서조화서불조용첨가Na2SeO3·5H2O 2 mg／kg적상규사료위양，미서조화미서불조용첨가MgSO4·7 H2O 162．5 mg／kg＋ Na2SeO35· H2O 2 mg／kg적상규사료위양。사양42 d ，제42천시관찰불아증적발생정황，병처사동물획취절아표본，H E염색관찰성유세포형태。결과가불조소서절아성유세포뉴곡변형，세포내출현공포，Tomes돌소실，불아증발병솔위100％；미서불조소서성유세포형태교가불조명현개선，불아증발병솔위40％，여가불조차이명현（ P＜0．01）。결론불대소서절아성유세포유독성작용，조성불아증，미서가길항기작용，감경불아증적병변정도。
Objective To study the antagonistic effects of Mg‐Se preparation on dental fluorosis of mice and its mechanism for providing the foundational data for the prevention control of dental fluorosis .Method 80 male SPF ICR mice ,randomly divided into 8 groups which were controlled group ,magnesium group ,selenium group ,magne‐sium‐selenium group ,fluoride group ,magnesium‐fluorine group ,selenium‐fluorine group and magnesium‐selenium‐fluorine group .The first four groups drank double steamed water ,the other four groups drank 50 mg/L F‐double steamed water .The controlled group and fluoride group fed conventionally .Magnesium group and magnesium‐fluo‐rine group fed conventionally by adding MgSO4 · 7 H2 O 162 .5 mg/kg .Selenium group and selenium‐fluorine group fed conventionally by adding Na2 SeO3 · 5 H2 O 2 mg/kg .magnesium‐selenium group and magnesium‐selenium‐fluo‐rine group fed conventionally by adding MgSO4 · 7 H2O 162 .5 mg/kg + Na2SeO3 · 5 H2O 2 mg/kg .The occur‐rences of mice dental fluorosis and final grade of dental fluorosis were observed when they were 42‐day .Then obtain incisor specimens after put the mice into death .And HE staining was used to observe the morphology of ameloblast . Result In the fluoride group some distorted cell and vacuoles could be found in the ameloblasts ,Tomes process in the ameloblasts disappeared .And the incidence of dental fluorosis was 100% .The morphology of ameloblast could im‐prove in the magnesium‐selenium‐fluorine group .The incidence of dental fluorosis was 40% .There were obvious differences between the fluoride group and the magnesium‐selenium‐fluorine group(P<0 .01) .Conclusion Fluoride has a toxic effect on the ameloblasts of the mice incisor that cause the dental fluorosis .But magnesium‐selenium has the antagonistic effect ,that cause the lesion degree was reduced .