CAJ | 학술논문

目的：探讨胃癌患者外周血中Survivin mRNA水平与幽门螺杆菌（ Hp）感染的关系。方法应用实时荧光定量qPCR技术检测胃炎及化疗前胃癌各50例患者外周血中Survivin mRNA表达，并采用快速尿素酶试验与Giemsa 染色2种方法检测Hp感染情况。结果胃炎患者外周血中未检测到Survivin mRNA表达；胃癌组患者Survivin表达检测率为84?0％（42／50），外周血Survivin mRNA表达水平的中位数为0?46。 Survivin表达水平与胃癌患者的年龄、性别及淋巴结转移均无关（ P＞0?05），而与TNM分期有关（ P＜0?0001）。胃癌患者Hp感染率为80?0％（40／50），显著高于胃炎患者的58?0％（29／50），差异有统计学意义（P＜0?05）。 Hp阳性的胃癌患者外周血Survivin mRNA表达水平为0?578±0?507，显著高于Hp阴性患者的0?194±0?419，差异有统计学意义（P＜0?05）。经Spearman相关分析，胃癌患者Survivin mRNA表达与Hp感染呈正相关（r＝0?464， P＜0?05）。结论 Hp感染及Survivin与胃癌发生可能有关，Survivin可能参与了Hp感染性胃癌的发展。
목적：탐토위암환자외주혈중Survivin mRNA수평여유문라간균（ Hp）감염적관계。방법응용실시형광정량qPCR기술검측위염급화료전위암각50례환자외주혈중Survivin mRNA표체，병채용쾌속뇨소매시험여Giemsa 염색2충방법검측Hp감염정황。결과위염환자외주혈중미검측도Survivin mRNA표체；위암조환자Survivin표체검측솔위84?0％（42／50），외주혈Survivin mRNA표체수평적중위수위0?46。 Survivin표체수평여위암환자적년령、성별급림파결전이균무관（ P＞0?05），이여TNM분기유관（ P＜0?0001）。위암환자Hp감염솔위80?0％（40／50），현저고우위염환자적58?0％（29／50），차이유통계학의의（P＜0?05）。 Hp양성적위암환자외주혈Survivin mRNA표체수평위0?578±0?507，현저고우Hp음성환자적0?194±0?419，차이유통계학의의（P＜0?05）。경Spearman상관분석，위암환자Survivin mRNA표체여Hp감염정정상관（r＝0?464， P＜0?05）。결론 Hp감염급Survivin여위암발생가능유관，Survivin가능삼여료Hp감염성위암적발전。
Objective To investigate the relationship between helicobacter pylori( Hp) infection and expression level of sur?vivin mRNA in the peripheral blood of gastric cancer patients. Methods The expression levels of survivin mRNA in the peripheral blood of gastric cancer and gastritis were detected by real?time quantitative reverse transcription polymerase chain reaction ( qPCR) . Hp infection was determined by rapid urease test and giemasa staining. Results In the specimens of gastritis patients and gastric cancer patients,the expression rates of survivin mRNA were 0 and 84?0%. Median value was 0?46 for expression of survivin mRNA in the pe?ripheral blood of gastric cancer patients. The expression levels of survivin mRNA were unrelated with age, gender and lymph node me?tastasis(P>0?05), but was significantly related with TNM stage(P<0?05). The positive expression rate of Hp in gastric cancer was 80?0%( 40/50 ) , significantly higher than 58?0%( 29/50 ) in gastritis patients ( P<0?05 ) . In the gastric cancer patients, the expression levels of survivin mRNA in Hp postive infection was 0?578±0?507, higher than 0?194±0?419 in Hp negative infection with significant differences ( P<0?05) . The expression survivin mRNA in gastric cancer patients and Hp infection was positively correlated( r=0?464, P<0?05) . Conclusion The expression of survivin and Hp infection may be correlated with gastric cancer. Survivin may par?ticipate in the progress of gastric cancer with Hp infection.