CAJ | 학술논문

目的：研究丙泊酚对自发性高血压鼠（SHR）大脑皮质细胞凋亡的影响。方法选择7周龄的SHR大鼠12只，随机分为两组：丙泊酚组（P组，n＝6），对照组（C组，n＝6）。分别于腹腔注射丙泊酚75 mg · kg －1及等容量生理盐水，对照组给予等容量的生理盐水，1次／d连续7d，第7d待丙泊酚组大鼠清醒后1h与对照组大鼠断头处死，并取左右大脑皮层组织，－80℃冰箱保存待检。用流式细胞术（FCA ）检测细胞凋亡率，Elisa法检测Caspase‐3含量。结果与C组比较，P组晚期细胞凋亡率和细胞死亡率显著降低[（32±6） VS （50±8），P＜0．01；（4．7±1．8） VS （9．3±1．5），P＜0．01）]；细胞存活率增加[（47±4） VS （36±8），P＜0．05）]；Caspase‐3含量[（34．4±2．1） VS （38．1±1．4） pmol／L ，P＜0．05）]降低。结论丙泊酚降低SHR大脑皮质Caspase‐3含量，减少晚期细胞凋亡率和死亡率，对SHR大鼠皮质细胞具保护作用。
목적：연구병박분대자발성고혈압서（SHR）대뇌피질세포조망적영향。방법선택7주령적SHR대서12지，수궤분위량조：병박분조（P조，n＝6），대조조（C조，n＝6）。분별우복강주사병박분75 mg · kg －1급등용량생리염수，대조조급여등용량적생리염수，1차／d련속7d，제7d대병박분조대서청성후1h여대조조대서단두처사，병취좌우대뇌피층조직，－80℃빙상보존대검。용류식세포술（FCA ）검측세포조망솔，Elisa법검측Caspase‐3함량。결과여C조비교，P조만기세포조망솔화세포사망솔현저강저[（32±6） VS （50±8），P＜0．01；（4．7±1．8） VS （9．3±1．5），P＜0．01）]；세포존활솔증가[（47±4） VS （36±8），P＜0．05）]；Caspase‐3함량[（34．4±2．1） VS （38．1±1．4） pmol／L ，P＜0．05）]강저。결론병박분강저SHR대뇌피질Caspase‐3함량，감소만기세포조망솔화사망솔，대SHR대서피질세포구보호작용。
Objective To investigate the effect of propofol on the apoptosis and the expression of caspase‐3 in the cortex cells of 7 weeks old spontaneously hypertensive rats (SHR) .Method 12 male SHR of 7 weeks old were ran‐domly divided into control group (n=6) and propofol group (n=6) and were intraperitoneally injected normal saline and propofol 75mg/kg q .d .For consecutive seven days .The animals were decapitated after the treatment ,the cor‐tex from both hemispheres were isolated and frozen at -80 ℃ until further analysis .The cortex cells were double stained with propidium iodide (PI) and annexin V‐FITC ,and then analyzed by flow cytometry .The quantitative de‐termination of caspase‐3 was measured using ELISA kits ,by spectrophotometrically at a wavelength of 450 nm ,re‐spectively .Result Early apoptotic cells are stained with annexin V‐FITC ,whereas late apoptotic cells were stained with both annexin V‐FITC and PI .The percentage of cells undergoing early apoptosis was increased in group P com‐pared with cells in group C [(15 .4 ± 4 .1) VS .(5 .1 ± 1 .8)] ,P<0 .01) .However ,the percentage of cells undergo‐ing late apoptosis was significantly decreased in group P compared with group C [(32 ± 6 ) VS .(50 ± 8 )] , P<0 .01) .There were statistically significant differences between the group P and group C for the level of caspase‐3 [(34 .4 ± 2 .1) VS .(38 .1 ± 1 .4)]pmol /L ,P <0 .05) .Conclusion Propofol attenuate late apoptosis and cells death in cerebral cortex of SHR ,the underlying mechanisms may be ameliorating the caspase‐3 activation .