CAJ | 학술논문

目的：分析血小板功能的影响因素，为建立和完善血小板功能分析的质量控制体系。方法2012年12月-2014年12月随机选取300名志愿者静脉血液，在不同血小板血浆量、血小板浓度、诱导剂浓度、血样放置时间、抗凝剂种类实验条件下，采用血浆比浊法进行血小板聚集率检测。结果血小板浓度1.0×1011/L以下时对血小板凝聚具有明显影响(P<0.05)；血小板聚集率随诱导剂浓度增高而增高，在AA为0.4 mmol/L时，聚集率最低，在从0.7 mmol/L血小板聚集率最高，之后随AA浓度增大到1.3 mmol/L时，聚集率由(72.9±13.4)降低到(66.7±15.6)，有降低的趋势；样本常温放置8h后血小板聚集率降低明显；枸橼酸对血小板凝血作用最明显。结论控制血小板功能试验前的各项影响指标，放置时间，血液样本的抗凝剂的选择，诱导剂的选择都需要严格注意，为保证实验结果的准确性。
목적：분석혈소판공능적영향인소，위건립화완선혈소판공능분석적질량공제체계。방법2012년12월-2014년12월수궤선취300명지원자정맥혈액，재불동혈소판혈장량、혈소판농도、유도제농도、혈양방치시간、항응제충류실험조건하，채용혈장비탁법진행혈소판취집솔검측。결과혈소판농도1.0×1011/L이하시대혈소판응취구유명현영향(P<0.05)；혈소판취집솔수유도제농도증고이증고，재AA위0.4 mmol/L시，취집솔최저，재종0.7 mmol/L혈소판취집솔최고，지후수AA농도증대도1.3 mmol/L시，취집솔유(72.9±13.4)강저도(66.7±15.6)，유강저적추세；양본상온방치8h후혈소판취집솔강저명현；구연산대혈소판응혈작용최명현。결론공제혈소판공능시험전적각항영향지표，방치시간，혈액양본적항응제적선택，유도제적선택도수요엄격주의，위보증실험결과적준학성。
Objective A Factor Analysis of platelet aggregation, for the establishment and improvement of platelet function analysis of the quality control system. Methods 300 volunteers venous blood, platelet-rich plasma at different volume, platelet concentration, inducer concentration, blood storage time, type and experimental conditions anticoagulants plasma, plasma assay was performed using platelet aggregation testing. Results Platelet concentration 1.0* 1011/L or less obvious effect on platelet aggregation(P<0 . 05); induced platelet aggregation with increased and increased concentration in AA0.4mmol/L, the aggregation rate lowest in from 0.7mmol/L platelet aggregation highest rate further increased with the AA concentration, platelet aggregation rate decreased; sample placed at room temperature for 8h platelet aggregation rate de-creased significantly; the most obvious citrate platelet clotting effect. Conclusion Control of platelet function tests before impact indicators, the accuracy of the experimental results has a crucial role.