CAJ | 학술논문

目的：探讨孕酮对东莨菪碱致大鼠认知功能障碍的影响。方法将健康雄性SD大鼠40只随机分为盐水对照组、东莨菪碱组、孕酮干预组和单纯孕酮组4组,每组10只。采用腹腔注射东莨菪碱的方法建立大鼠认知功能障碍模型。东莨菪碱组腹腔注射东莨菪碱1.8 mg/kg,孕酮干预组腹腔注射东莨菪碱1.8 mg/kg和孕酮8 mg/ kg,单纯孕酮组腹腔注射等容量的孕酮8 mg/kg和生理盐水,盐水对照组腹腔注射等容量的生理盐水。于注药后第7天行Y-型电迷宫实验,观察大鼠认知功能变化。 Y-型电迷宫实验完毕即刻处死大鼠,取海马组织,采用HE染色法观察海马组织的形态学变化,采用免疫组化法测定海马组织谷氨酸( Glu)和γ-氨基丁酸( GABA)的含量。结果与盐水对照组比较,东莨菪碱组与孕酮干预组注药后第7天 Y-型电迷宫实验中大鼠总训练次数增加、达标时间延长(P <0．05),Y-型电迷宫实验后大鼠海马组织Glu含量升高、GABA含量降低(P<0．05),海马组织病理损伤程度加重；单纯孕酮组上述指标与盐水对照组比较差异无统计学意义(P>0.05)。与东莨菪碱组比较,孕酮干预组注药后第7天Y-型电迷宫实验中大鼠总训练次数减少、达标时间缩短(P<0．05),海马组织病理损伤程度减轻,Y-型电迷宫实验后大鼠海马组织Glu含量降低、GABA含量升高(P<0．05)。结论腹腔注射孕酮可改善东莨菪碱所致大鼠的认知功能障碍,其机制可能与孕酮可调节海马组织Glu和γ-GABA的含量有关。
목적：탐토잉동대동랑탕감치대서인지공능장애적영향。방법장건강웅성SD대서40지수궤분위염수대조조、동랑탕감조、잉동간예조화단순잉동조4조,매조10지。채용복강주사동랑탕감적방법건립대서인지공능장애모형。동랑탕감조복강주사동랑탕감1.8 mg/kg,잉동간예조복강주사동랑탕감1.8 mg/kg화잉동8 mg/ kg,단순잉동조복강주사등용량적잉동8 mg/kg화생리염수,염수대조조복강주사등용량적생리염수。우주약후제7천행Y-형전미궁실험,관찰대서인지공능변화。 Y-형전미궁실험완필즉각처사대서,취해마조직,채용HE염색법관찰해마조직적형태학변화,채용면역조화법측정해마조직곡안산( Glu)화γ-안기정산( GABA)적함량。결과여염수대조조비교,동랑탕감조여잉동간예조주약후제7천 Y-형전미궁실험중대서총훈련차수증가、체표시간연장(P <0．05),Y-형전미궁실험후대서해마조직Glu함량승고、GABA함량강저(P<0．05),해마조직병리손상정도가중；단순잉동조상술지표여염수대조조비교차이무통계학의의(P>0.05)。여동랑탕감조비교,잉동간예조주약후제7천Y-형전미궁실험중대서총훈련차수감소、체표시간축단(P<0．05),해마조직병리손상정도감경,Y-형전미궁실험후대서해마조직Glu함량강저、GABA함량승고(P<0．05)。결론복강주사잉동가개선동랑탕감소치대서적인지공능장애,기궤제가능여잉동가조절해마조직Glu화γ-GABA적함량유관。
Objective To investigate the effect of Progesterone on Scopolamine-induced postoperative cognitive dysfunction ( POCD) in rats. Methods A total of 40 healthy male SD rats were randomly divided into saline control group ( n=10 ) , Scopolamine group ( n=10 ) , Progesterone intervention group ( n=10 ) and pure Progesterone group (n=10). The rat models of cognitive dysfunction were established taking intraperitoneal injection of Scopolamine. Sco-polamine group was intraperitoneally injected with Scopolamine (1. 8 mg/kg);Progesterone intervention group was intra-peritoneally injected with Scopolamine (1. 8 mg/kg) and Progesterone (8 mg/kg);pure Progesterone group was intraper-itoneally injected with equal volume of Progesterone (8 mg/kg) and physiological saline;saline control group was intrap-eritoneally injected with equal volume of physiological saline. The Y-type maze test was performed in the 4 groups on the 7th d after injection to observe the changes of the rats'cognitive function. After the Y-type electric maze test, the rats were killed immediately, and the hippocampal neurons were taken. Hematoxylin eosin staining ( HE staining) method was used to observe the morphological changes, and immunohistochemical method was used to detect the contents of glutamic acid ( GLU) and gamma-aminobutyric acid ( GABA) . Results Compared with those in saline control group, the fre-quency of total training was increased, and the time to meet the standard time was prolonged on the 7th d after injection during the Y-type electric maze test in Scopolamine and Progesterone intervention groups (P<0. 05);after the Y-type electric maze test, the GLU content was increased, and the GABA content was decreased in hippocampal neurons ( P<0. 05), and the pathological injuries were aggravated;while the differences of above indexes in pure Progesterone group were not statistically significant (P>0. 05). Compared with those in Scopolamine group, the frequency of total training was reduced, and the time to meet the standard time was shortened on the 7th d after injection during the Y-type electric maze test in Progesterone intervention group (P<0. 05), and the pathological injuries were alleviated;after the Y-type electric maze test, the GLU content was decreased, and the GABA content was increased in hippocampal neurons ( P<0. 05). Conclusion Intraperitoneal injection of Progesterone may improve Scopolamine-induced postoperative cognitive dysfunction, and its mechanisms are probably related to regulating the contents of GLU and GABA in hippocampal neu-rons by Progesterone.