CAJ | 학술논문

目的：通过对髁突软骨早期生长阶段缺氧位置及其时间依赖性的研究，探讨其在髁突发育中的意义。方法：40只14 d 龄 SD 大鼠哺乳喂养，第21天断奶喂正常食物，分别于断奶后12、24、48以及96 h 各处死10只，并于处死前2 h 腹腔注射盐酸哌莫硝唑（HP-1）60 mg／kg，取完整髁突组织，用免疫组化方法检测 HP-1的表达。结果：HP-1主要表达在髁突软骨表面纤维层、增殖层软骨细胞，且主要位于关节负重区，即髁突前斜面和关节结节后斜面。半定量分析表明，髁突软骨 HP －1的表达24 h 时高于12、48、96 h（P ＜0．01），其他时间点之间差异均无统计学意义（P ＞0．05）。结论：早期生长阶段，食物负荷可能直接导致髁突软骨缺氧，缺氧位于软骨上层且呈时间依赖性改变。
목적：통과대과돌연골조기생장계단결양위치급기시간의뢰성적연구，탐토기재과돌발육중적의의。방법：40지14 d 령 SD 대서포유위양，제21천단내위정상식물，분별우단내후12、24、48이급96 h 각처사10지，병우처사전2 h 복강주사염산고막초서（HP-1）60 mg／kg，취완정과돌조직，용면역조화방법검측 HP-1적표체。결과：HP-1주요표체재과돌연골표면섬유층、증식층연골세포，차주요위우관절부중구，즉과돌전사면화관절결절후사면。반정량분석표명，과돌연골 HP －1적표체24 h 시고우12、48、96 h（P ＜0．01），기타시간점지간차이균무통계학의의（P ＞0．05）。결론：조기생장계단，식물부하가능직접도치과돌연골결양，결양위우연골상층차정시간의뢰성개변。
Objective:To investigate hypoxia location in condylar cartilage in the early growth stage of rats.Methods:40 Sprague-Dawley rats were breastfed from 1 4 d to 21 d of age.1 0 rats were sacrificed at 1 2,24,48 and 96 h respectively after initiation of normal food at 21 d of age.The rats were administered pimonidazole hydrochloride (HP-1 )at a dose of 60 mg/kg by intraperitoneal injection 2 h before sacrifice.The expression of HP-1 in the whole condylar cartilage was detected by immunohistochemical staining.Results:HP-1 was mainly expressed in the chondrocytes of the fibrous and proliferative layer of cartilage,primarily concentrated in the weight-bearing area of joint-anterior aspect of the condyle and posterior aspect of the articular eminence at all time points.The highest expres-sion was observed at 24 h after initiation of normal food (P <0.01 ).Conclusion:In the early growth stage of rats,dietary loading may directly induce hypoxia in uper layer of condylar cartilage,the hyoxia level may change with time of dietary loading.