CAJ | 학술논문

目的：探讨荧光原位杂交（ FISH）检测乳腺癌表皮生长因子受体2（ HER2）基因扩增在淮海经济区的推广和应用。方法通过组织芯片技术，运用免疫组织化学（ IHC）法和FISH分别检测124例浸润性乳腺癌组织中HER2蛋白表达和HER2基因扩增状况，并进行对比分析。结果124例乳腺癌中HER2蛋白表达为（眓眓眓）39例，（眓眓）70例，（0，＋）15例，其FISH阳性率分别为87．18％、24．29％、20．00％，FISH法与IHC法检测结果总体符合率为85．19％。结论在淮海经济区，采用IHC检测Her2蛋白表达为（眓眓）时与Her2基因扩增状态存在较大差异，IHC呈阳性患者有必要选择性地进行FISH检测，有助于指导临床治疗及判断患者预后。
목적：탐토형광원위잡교（ FISH）검측유선암표피생장인자수체2（ HER2）기인확증재회해경제구적추엄화응용。방법통과조직심편기술，운용면역조직화학（ IHC）법화FISH분별검측124례침윤성유선암조직중HER2단백표체화HER2기인확증상황，병진행대비분석。결과124례유선암중HER2단백표체위（활활활）39례，（활활）70례，（0，＋）15례，기FISH양성솔분별위87．18％、24．29％、20．00％，FISH법여IHC법검측결과총체부합솔위85．19％。결론재회해경제구，채용IHC검측Her2단백표체위（활활）시여Her2기인확증상태존재교대차이，IHC정양성환자유필요선택성지진행FISH검측，유조우지도림상치료급판단환자예후。
Objective To discuss the promotion and application of fluorescence in situ hybridization ( FISH) to de-tect the amplification of human epithelial growth factor receptor 2 (HER2) gene in breast cancer patients of Huaihai eco-nomic zone.Methods A total of 124 human breast cancer samples were prepared by tissue microarray ( TMA) .Their expression of HER2 at protein and mRNA levels was determined by immunohistochemistry ( IHC) and FISH.Then, both methods were compared for their consistency.Results Among the samples were 39 HER2 strongly positive samples (艹+), 70 positive ones (艹), and 15 weak positive or negative ones (0/+), according to IHC staining.Using FISH, the corresponding positive rates of HER2 gene were 87.18%, 24.29%, and 20.00%respectively, which showed 85.19%of consistence with IHC results.Conclusion There is a big difference between the expression of HER2 protein (艹) de-tected by IHC and gene amplification in the Huaihai economic zone.HER2 positive carriers are necessary to undergo FISH in order to improve clinical treatment and prognosis.