CAJ | 학술논문

目的：探讨在髁突软骨细胞受到周期性单轴压力后，结缔组织生长因子（connective tissue growth factor，CTGF）表达的影响，为正畸治疗中髁突软骨受力后的改建提供生物学依据。方法选取1周龄SD大鼠，提取并培养髁突软骨细胞，免疫组化鉴定。利用四点弯曲细胞力学加载仪对第3代细胞进行力值为2000u strain、0.5Hz的体外周期性单轴压力加载，分别在加力0min、30min、60min和120min后继续培养24h，应用蛋白印迹法检测在不同加力时间CTGF蛋白表达的变化。应用SPSS18.0软件对数据进行统计学分析。结果 CTGF的相对蛋白量在加力0min、30min、60min和120min后，分别为0、1.59、2.34和3.16，随着加载时间的增加，表达呈逐渐上升趋势。且组间差异具有统计学意义（P＜0.05）结论周期性单轴压力可刺激大鼠髁突软骨细胞CTGF的表达。
목적：탐토재과돌연골세포수도주기성단축압력후，결체조직생장인자（connective tissue growth factor，CTGF）표체적영향，위정기치료중과돌연골수력후적개건제공생물학의거。방법선취1주령SD대서，제취병배양과돌연골세포，면역조화감정。이용사점만곡세포역학가재의대제3대세포진행력치위2000u strain、0.5Hz적체외주기성단축압력가재，분별재가력0min、30min、60min화120min후계속배양24h，응용단백인적법검측재불동가력시간CTGF단백표체적변화。응용SPSS18.0연건대수거진행통계학분석。결과 CTGF적상대단백량재가력0min、30min、60min화120min후，분별위0、1.59、2.34화3.16，수착가재시간적증가，표체정축점상승추세。차조간차이구유통계학의의（P＜0.05）결론주기성단축압력가자격대서과돌연골세포CTGF적표체。
Object To investigate the effect of cyclic compressive stress on the expression of connective tissue growth factor (CTGF) in rat mandibular condylar Chondrocytes. Methods Condyle chondrocytes were isolated from rat condylar cartilage. The third generation of Mandibulae Condyle chondrocytes were subjected to 0.5Hz frequency with 2000u strain strength for 0, 30, 60 and 120 min using a Four Point Bending system. The cells were then collected after 24 hours. The expression of CTGF was detected by western blot. All data obtained were analyzed by SPSS 18.0. Results The relative amount of protein CTGF after 0 min, 30 min, 60 min and 120 min of cyclic compressive stress loading were respectively 0, 1.59, 2.34, and 3.16. With the increasing time of cyclic compressive stress loading, the expression of CTGF increased. There were significant differences among those groups (P<0.05). Conclusions Cyclic Compressive Stress induced the increased expression of CTGF in rat mandibular condylar Chondrocytes. As a cytokine, CTGF may mediate the morphological changes by cyclic compressive stress in condylar cartilage.