CAJ | 학술논문

为建立黑木相思(Acacia melanoxylon)快繁技术体系，以含1个腋芽的无菌茎段为材料，研究了植物生长调节剂对其增殖和生根的影响。结果表明，6-BA极易诱导黑木相思愈伤组织形成，但芽长势较差，不利于腋芽增殖体系的建立。而生长素既能诱导黑木相思生根，又能诱导腋芽增殖；将无菌茎段接入MS+IAA 0.5 mg L–1+IBA 0.5 mg L–1培养基中培养20 d的生根率为98.41%，培养40 d的腋芽增殖倍数为2.36，单株繁殖系数为6.57。这是首次成功建立高效、简便的生根和增殖同步发生的黑木相思直接器官发生途径的组培技术体系。
위건립흑목상사(Acacia melanoxylon)쾌번기술체계，이함1개액아적무균경단위재료，연구료식물생장조절제대기증식화생근적영향。결과표명，6-BA겁역유도흑목상사유상조직형성，단아장세교차，불리우액아증식체계적건립。이생장소기능유도흑목상사생근，우능유도액아증식；장무균경단접입MS+IAA 0.5 mg L–1+IBA 0.5 mg L–1배양기중배양20 d적생근솔위98.41%，배양40 d적액아증식배수위2.36，단주번식계수위6.57。저시수차성공건립고효、간편적생근화증식동보발생적흑목상사직접기관발생도경적조배기술체계。
In order to establish rapid propagation system of Acacia melanoxylon, the effects of plant growth regulators on its axillary bud multiplication and rooting were studied by using sterile stem segments with one axillary bud as explants. The results showed that the calli of A. melanoxylon were easily induced and bud growth was poor by 6-BA, indicating the disadvantage of axillary bud proliferation. The proliferation and rooting of Acacia melanoxylon axillary buds could be induced by auxin. The stem sections were cultured on MS+IAA 0.5 mg L–1+IBA 0.5 mg L–1 medium, rooting rate was 98.41%cultured for 20 d, and cultured for 40 d, the axillary bud multiplication and propagation coefficient were 2.36 and 6.57 per plantlet, respectively. It was successfully established the direct organogenesis technology system of A. melanoxylon for the first time with the advantages of high efficient, easy rooting and proliferation occur synchronous.