CAJ | 학술논문

目的：研究血清TGF-β1以及BMP-7在呼吸窘迫综合征新生患儿中的表达趋势。方法在该院随机选取2014年2月-2015年2月30例没有使用PS的NRDS早产婴儿作为非PS组，30例出生后12 h内给予肺表面的活性物质的NRDS早产婴儿作为PS组；选取无NRDS的30例早产婴儿作为对照组。分别在出生后各时间点用ELISA检验3组早产患儿血清中的TGF-β1以及BMP-7的表达情况。结果非PS组血清TGF-β1表达量各时间点显著高于对照组(P<0.05)，后3、7 d时间内显著高于PS组(P<0.05)，PS组的血清TGF-β1表达量在出生后的1、3 d时间内显著高于对照组(P<0.05)；非PS组血清BMP-7表达量各时间点高于对照组，在出生后7 d BMP-7的表达量有所下降，仍高PS组(P<0.05)，PS组血清BMP-7表达量在出生后1、3 d明显高于对照组(P<0.05)。结论NRDS早产患儿的血清TGF-β1以及BMP-7在表达量上均有所增加，后期会随着血清TGF-β1表达增加，BMP-7的表达趋势下降，以此提示外源性的BMP-7会降低TGF-β1的表达。
목적：연구혈청TGF-β1이급BMP-7재호흡군박종합정신생환인중적표체추세。방법재해원수궤선취2014년2월-2015년2월30례몰유사용PS적NRDS조산영인작위비PS조，30례출생후12 h내급여폐표면적활성물질적NRDS조산영인작위PS조；선취무NRDS적30례조산영인작위대조조。분별재출생후각시간점용ELISA검험3조조산환인혈청중적TGF-β1이급BMP-7적표체정황。결과비PS조혈청TGF-β1표체량각시간점현저고우대조조(P<0.05)，후3、7 d시간내현저고우PS조(P<0.05)，PS조적혈청TGF-β1표체량재출생후적1、3 d시간내현저고우대조조(P<0.05)；비PS조혈청BMP-7표체량각시간점고우대조조，재출생후7 d BMP-7적표체량유소하강，잉고PS조(P<0.05)，PS조혈청BMP-7표체량재출생후1、3 d명현고우대조조(P<0.05)。결론NRDS조산환인적혈청TGF-β1이급BMP-7재표체량상균유소증가，후기회수착혈청TGF-β1표체증가，BMP-7적표체추세하강，이차제시외원성적BMP-7회강저TGF-β1적표체。
Objective To study the expression trend of serum TGF-β1 and BMP-7 in newborns with respiratory distress syndrome. Methods From February 2014 to February 2015, 30 NRDS premature infants without using PS were selected as the non-PS group, 30 NRDS premature infants treated by PS within 12 hours after birth were selected as the PS group; and other 30 premature infants without NRDS were set as the control group. ELISA was used to detect the expression of serum TGF-β1 and BMP-7 in the three groups at different time points after birth. Results The expression of serum TGF-β1 of non-PS group at different time points after birth was significantly higher than that of the control group, respectively (P<0.05), and significantly higher than that of the PS group 3d, 7d after birth (P<0.05). The expression of serum TGF-β1 of the PS group 1d, 3d after birth was significantly higher than that of the control group (P<0.05). The expression of serum BMP-7 of non-PS group at different time points after birth was significantly higher than that of the control group, respectively; al-though the expression of BMP-7 of non-PS group decreased 7d after birth, it was higher than that of the PS group (P<0.05). The expression of serum BMP-7 of PS group was much higher than that of the control group 1d, 3d after birth (P<0.05). Conclusion The expressions of serum TGF-β1 and BMP-7 in NRDS premature infants increased, but with the increase of serum TGF-β1, the expression of BMP-7 decreased at later time, which indicates that exogenous BMP 7 would reduce the expression of TGF-β1.