中南医学科学杂志
中南醫學科學雜誌
중남의학과학잡지
Medical Science Journal of Central South China
2015年
6期
601-604,674
,共5页
杨瑞瑞%向梦琴%周秀田%扶云%徐海帆%陈卓%游琴%王佩华%曾敏%徐迎晖%曾希
楊瑞瑞%嚮夢琴%週秀田%扶雲%徐海帆%陳卓%遊琴%王珮華%曾敏%徐迎暉%曾希
양서서%향몽금%주수전%부운%서해범%진탁%유금%왕패화%증민%서영휘%증희
胃癌%血清标志物%miR-20a%实时定量PCR
胃癌%血清標誌物%miR-20a%實時定量PCR
위암%혈청표지물%miR-20a%실시정량PCR
gastric cancer%serum biomarker%miR-20a%qRT-PCR
目的：探讨胃癌初诊患者血清miR-20a的表达情况及其与临床病理特征的关系。方法采集45例原发胃癌初诊患者血清，应用实时定量PCR(qRT-PCR)，加入cel-miR-39作为数据标准化指标，检测血清miR-20a的表达量。结果胃癌患者血清中miR-20a表达与胃癌分化程度、TNM分期及淋巴结转移密切相关(P<0.05)。低分化组血清miR-20a表达水平明显高于中分化组和高分化组。 miR-20a在Ⅱ、Ⅲ、Ⅳ期患者血清中的表达水平明显高于Ⅰ期，有淋巴结转移组明显高于无淋巴结转移组。 miR-20a的表达与患者的性别、年龄无明显相关性。结论胃癌患者血清miR-20a表达与胃癌分化程度、临床分期及淋巴结转移相关，可能用于胃癌早期诊断和病情监测。
目的：探討胃癌初診患者血清miR-20a的錶達情況及其與臨床病理特徵的關繫。方法採集45例原髮胃癌初診患者血清，應用實時定量PCR(qRT-PCR)，加入cel-miR-39作為數據標準化指標，檢測血清miR-20a的錶達量。結果胃癌患者血清中miR-20a錶達與胃癌分化程度、TNM分期及淋巴結轉移密切相關(P<0.05)。低分化組血清miR-20a錶達水平明顯高于中分化組和高分化組。 miR-20a在Ⅱ、Ⅲ、Ⅳ期患者血清中的錶達水平明顯高于Ⅰ期，有淋巴結轉移組明顯高于無淋巴結轉移組。 miR-20a的錶達與患者的性彆、年齡無明顯相關性。結論胃癌患者血清miR-20a錶達與胃癌分化程度、臨床分期及淋巴結轉移相關，可能用于胃癌早期診斷和病情鑑測。
목적：탐토위암초진환자혈청miR-20a적표체정황급기여림상병리특정적관계。방법채집45례원발위암초진환자혈청，응용실시정량PCR(qRT-PCR)，가입cel-miR-39작위수거표준화지표，검측혈청miR-20a적표체량。결과위암환자혈청중miR-20a표체여위암분화정도、TNM분기급림파결전이밀절상관(P<0.05)。저분화조혈청miR-20a표체수평명현고우중분화조화고분화조。 miR-20a재Ⅱ、Ⅲ、Ⅳ기환자혈청중적표체수평명현고우Ⅰ기，유림파결전이조명현고우무림파결전이조。 miR-20a적표체여환자적성별、년령무명현상관성。결론위암환자혈청miR-20a표체여위암분화정도、림상분기급림파결전이상관，가능용우위암조기진단화병정감측。
Objective To investigate the relationship between serum miR-20a expression and clinicopathological fea-tures in gastric cancer. Methods Serum was collected in 45 primary gastric cancer patients who were untreated. Real-time quantitative PCR was adopted to detect the expression of miR-20a in serum using C. elegans miR-39 as an internal reference gene. Results In this case,we analyzed the relationship between the serum miR-20a expression level and clinicopathologi-cal features. The results showed that expression of miR-20a was related with tumor differentiation,TNM stages and lymph node metastasis (P<0. 05). The expression level of serum miR-20a was significantly higher in poorly differentiated gastric cancer patients than that in moderately differentiated and well differentiated gastric cancer patients. Moreover,miR-20a level was sig-nificantly higher in stageⅡ,ⅢandⅢthan stageⅠ, also higher in lymph node metastasis group than those patients without lymph node metastasis. Conclusion The expression level of miR-20a is related with tumor differentiation,TNM stages and lymph node metastasis. MiR-20a is a candidate biomarker for detecting and monitoring gastric cancer.
