中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
10期
122-126
,共5页
俞安安%袁芳%杨智承%夏自花%范凯燕%杨帆
俞安安%袁芳%楊智承%夏自花%範凱燕%楊帆
유안안%원방%양지승%하자화%범개연%양범
拉莫三嗪%微透析%体外回收率%体内回收率
拉莫三嗪%微透析%體外迴收率%體內迴收率
랍막삼진%미투석%체외회수솔%체내회수솔
lamotrigine%microdialysis%in vitro recovery%in vivo recovery
目的 建立高效液相色谱(high performance liquid chromatography,HPLC)测定血管微透析样品中拉莫三嗪含量的方法,测定拉莫三嗪微透析体外回收率并考察其影响因素,筛选适用于体内实验的微透析条件.方法 采用正、反透析法测定拉莫三嗪血管微透析的体外回收率,并考察流速、浓度、温度、时间对回收率的影响,验证反透析法用于测定体内回收率的可行性并筛选出体内实验的最佳灌流速度和平衡时间;采用反透析法测定血管探针的体内回收率并考察其体内稳定性.结果 在相同条件下探针相对回收率与相对损失率近似相等,并随流速增加而降低,随温度升高而增高;在相同流速下,回收率与药物浓度无关;当灌流速度为2μL/min,平衡时间为1.5 h时,探针的体内回收率可维持6.5 h稳定.结论 微透析采样技术可用于拉莫三嗪的血药浓度研究,反透析法可作为拉莫三嗪血管微透析体内回收率的测定方法.
目的 建立高效液相色譜(high performance liquid chromatography,HPLC)測定血管微透析樣品中拉莫三嗪含量的方法,測定拉莫三嗪微透析體外迴收率併攷察其影響因素,篩選適用于體內實驗的微透析條件.方法 採用正、反透析法測定拉莫三嗪血管微透析的體外迴收率,併攷察流速、濃度、溫度、時間對迴收率的影響,驗證反透析法用于測定體內迴收率的可行性併篩選齣體內實驗的最佳灌流速度和平衡時間;採用反透析法測定血管探針的體內迴收率併攷察其體內穩定性.結果 在相同條件下探針相對迴收率與相對損失率近似相等,併隨流速增加而降低,隨溫度升高而增高;在相同流速下,迴收率與藥物濃度無關;噹灌流速度為2μL/min,平衡時間為1.5 h時,探針的體內迴收率可維持6.5 h穩定.結論 微透析採樣技術可用于拉莫三嗪的血藥濃度研究,反透析法可作為拉莫三嗪血管微透析體內迴收率的測定方法.
목적 건립고효액상색보(high performance liquid chromatography,HPLC)측정혈관미투석양품중랍막삼진함량적방법,측정랍막삼진미투석체외회수솔병고찰기영향인소,사선괄용우체내실험적미투석조건.방법 채용정、반투석법측정랍막삼진혈관미투석적체외회수솔,병고찰류속、농도、온도、시간대회수솔적영향,험증반투석법용우측정체내회수솔적가행성병사선출체내실험적최가관류속도화평형시간;채용반투석법측정혈관탐침적체내회수솔병고찰기체내은정성.결과 재상동조건하탐침상대회수솔여상대손실솔근사상등,병수류속증가이강저,수온도승고이증고;재상동류속하,회수솔여약물농도무관;당관류속도위2μL/min,평형시간위1.5 h시,탐침적체내회수솔가유지6.5 h은정.결론 미투석채양기술가용우랍막삼진적혈약농도연구,반투석법가작위랍막삼진혈관미투석체내회수솔적측정방법.
Objective To establish a high performance liquid chromatography method to detect the concentration of lamotrigine in blood dialysate and investigate in vitro recovery of lamotrigine and the factors.Select the microdialysis conditions that apply to the animal experiment and guide the stability study of in vivo recovery.Methods Positive dialysis and retrodialysis were used for the examination of lamotrigine in vitro recovery and the influencing factors such as flow rate, concentration, temperature and time.Filtered out the best conditions that apply to the in vivo experiment.Used the retrodialysis to determine the in vivo recovery and its stability.Results There was no significant difference between relative recovery and relative loss in the same flow rate.The concentration had no obvious effect on relative recovery.At the same condition,relative recovery decreased with the increase of the flow rate and increased with the temperature.The in vivo recovery had a good stability of 6.5 hours when the flow rate and stabilization time were set at 2μL/min and 1.5 h, respectivily.Conclusion Microdialysis technique can be used for the pharmacokinetic study of lamotrigine.Retrodialysis can be used for the determination of the lamotrigine in vivo recovery.
