CAJ | 학술논문

目的:建立同时测定甘松中绿原酸、甘松新酮含量的测定方法,测定不同产地甘松中绿原酸和甘松新酮的含量,以控制其质量.方法:采用高效液相色谱法,色谱柱为Agilent Zorbax Eclipse Plus C18(250mm ×4.6mm,5μm),以乙腈-水(含0.1%磷酸)为流动相,梯度洗脱,流速为1.0mL/min,柱温为25℃,进样量为10μL,检测波长为274nm.结果:绿原酸、甘松新酮的浓度与峰面积呈良好的线性关系,其线性范围分别是0.1278~3.1954μg(r=0.9993,n=6)、0.3546~8.8654μg(r=1,n=6),平均回收率分别为99.2%,101.1%,RSD分别为1.73%,1.10%.结论:本法方便、准确、重现性好,可用于甘松药材中绿原酸和甘松新酮的含量测定.
목적:건립동시측정감송중록원산、감송신동함량적측정방법,측정불동산지감송중록원산화감송신동적함량,이공제기질량.방법:채용고효액상색보법,색보주위Agilent Zorbax Eclipse Plus C18(250mm ×4.6mm,5μm),이을정-수(함0.1%린산)위류동상,제도세탈,류속위1.0mL/min,주온위25℃,진양량위10μL,검측파장위274nm.결과:록원산、감송신동적농도여봉면적정량호적선성관계,기선성범위분별시0.1278~3.1954μg(r=0.9993,n=6)、0.3546~8.8654μg(r=1,n=6),평균회수솔분별위99.2%,101.1%,RSD분별위1.73%,1.10%.결론:본법방편、준학、중현성호,가용우감송약재중록원산화감송신동적함량측정.
Objective:To establish an HPLC method for the simultaneous determination of chlorogenic acid,nar-dosinone in Nardostachys chinensis DC.from different producing areas, so as to set up a sensitive and specific method for controlling its quality.Methods:The analysis was performed on an Agilent Zorbax Eclipse Plus C18 column(250mm ×4.6mm,5μm)with the gradient elution of acetonitrile-water (0.1% phosphonic acid) at the flowing rate of 1.0mL/min.The column temperature was at 25℃.and the volume of sample injection was 10μL,and the detection wavelength was set as 274mm.Results:Good linearity was obtained for Chlorogenic acid and nardosinone in the range of 0.1278~3.1954μg(r=0.9993,n=6)0.3546~8.8654μg(r=1,n=6) respectivelies.The average recovery rates of Chlorogenic acid, nardosinone and cryptotashinone were 99.2%,101.1%,respectively.Conclusion:The developed HPLC method is simple,accurate and repeatable, which is suitable for the determination of chlorogenic acid,nardosinone in Nardostachys chinensis DC.