中医药信息
中醫藥信息
중의약신식
Information on Traditional Chinese Medicine
2015年
6期
31-33
,共3页
高效液相色谱%双黄连粉针剂%黄芩苷
高效液相色譜%雙黃連粉針劑%黃芩苷
고효액상색보%쌍황련분침제%황금감
High performance liquid chromatography%Shuanghuanglian powder for injection%Baicalin
目的:建立一种双黄连粉针剂中黄芩苷的含量测定方法,用于控制双黄连粉针剂的质量.方法:采用岛津LC-20A高效液相色谱仪和Shim-Pack C18色谱柱(150mm ×4.6mm I.D.,5μm);流动相为乙腈-0.5%冰醋酸水溶液,梯度洗脱;流速1.0 mL/min;二极管阵列检测,检测波长为280 nm;柱温:30℃.结果:方法的专属性良好,黄芩苷在浓度 4μg/mL ~20μg/mL 范围内,线性关系良好(r =0.9998),日内精密度RSD=0.24%,n=5,日间精密度RSD=0.43%,n=3,黄芩苷的定量限为2μg/mL,检测限为0.67μg/mL,平均回收率100.23%,回收率RSD=1.95%,n=9.结论:建立了一种专属性强,精密度高,回收率良好的用于双黄亮粉针剂中黄芩苷含量测定的高效液相色谱分析方法.
目的:建立一種雙黃連粉針劑中黃芩苷的含量測定方法,用于控製雙黃連粉針劑的質量.方法:採用島津LC-20A高效液相色譜儀和Shim-Pack C18色譜柱(150mm ×4.6mm I.D.,5μm);流動相為乙腈-0.5%冰醋痠水溶液,梯度洗脫;流速1.0 mL/min;二極管陣列檢測,檢測波長為280 nm;柱溫:30℃.結果:方法的專屬性良好,黃芩苷在濃度 4μg/mL ~20μg/mL 範圍內,線性關繫良好(r =0.9998),日內精密度RSD=0.24%,n=5,日間精密度RSD=0.43%,n=3,黃芩苷的定量限為2μg/mL,檢測限為0.67μg/mL,平均迴收率100.23%,迴收率RSD=1.95%,n=9.結論:建立瞭一種專屬性彊,精密度高,迴收率良好的用于雙黃亮粉針劑中黃芩苷含量測定的高效液相色譜分析方法.
목적:건립일충쌍황련분침제중황금감적함량측정방법,용우공제쌍황련분침제적질량.방법:채용도진LC-20A고효액상색보의화Shim-Pack C18색보주(150mm ×4.6mm I.D.,5μm);류동상위을정-0.5%빙작산수용액,제도세탈;류속1.0 mL/min;이겁관진렬검측,검측파장위280 nm;주온:30℃.결과:방법적전속성량호,황금감재농도 4μg/mL ~20μg/mL 범위내,선성관계량호(r =0.9998),일내정밀도RSD=0.24%,n=5,일간정밀도RSD=0.43%,n=3,황금감적정량한위2μg/mL,검측한위0.67μg/mL,평균회수솔100.23%,회수솔RSD=1.95%,n=9.결론:건립료일충전속성강,정밀도고,회수솔량호적용우쌍황량분침제중황금감함량측정적고효액상색보분석방법.
Objective:To develop an quantitative HPLC method for the determination of baicalin in Shuanghuan-glian powder for injection.Methods: The HPLC analysis was carried out on LC-20A HPLC and a Shim-Pack C18 column(150 mm ×4.6mm I.D.,5μm) was used, with a gradient elution of acetonitrile-0.5%gla-cial acetic acid solution at the flow rate of 1.0mL/min.DAD was detected and the detective wave length was set at 280nm, and the column temperature was at 30℃.Results:The specificity of this method was good,and the calibration curve was linear within the range of 4~20μg/mL and for baicalin ( r=0.9998) .The intra-day precision was RSD 0.24%, n=5.The inter-day precision was RSD 0.43%, n=3.The Quantitative limit of baicalin was 2μg/mL,and the detection limit of baicalin was 0.67μg/mL.The average recovery for the baicalin was 100.23%(RSD=1.95%).Conclusion:With a good specificity, high precision and well re-covery the HPLC method was developed to determine the baicalin in Shuanghuanglian powder for injection.
