CAJ | 학술논문

目的:考察对羟基苯乙腈的急性毒性及其镇咳、祛痰作用.方法:从炒白芥子中分离纯化对羟基苯乙腈.采用改良寇氏法确定小鼠灌胃给药的LD50.以25%的浓氨水诱发实验动物咳、痰,以实验动物的咳嗽潜伏期、2min内咳嗽次数、气管酚红排痰量为评价指标,观察对羟基苯乙腈的镇咳、祛痰作用.结果:对羟基苯乙腈的半数致死量为288.3mg/kg.对羟基苯乙腈低、中、高剂量组实验动物的咳嗽潜伏期分别为(16.67±5.70)s、(20.17±4.93)s、(23.45±6.53)s,均长于阴性对照组实验动物的咳嗽潜伏期(14.53±3.86)s;与阳性对照组相比,组间差异不显著.对羟基苯乙腈低、中、高剂量组实验动物2min内咳嗽次数分别为(34.77±11.47)次、(24.95±7.46)次、(26.25±7.68)次,均少于阴性对照组(43.76±9.49)次,且与阳性对照组相比,组间差异不显著.低、中、高剂量对羟基苯乙腈均可提高痰液分泌量.结论:对羟基苯乙腈具有一定的毒性且镇咳、祛痰作用明显.
목적:고찰대간기분을정적급성독성급기진해、거담작용.방법:종초백개자중분리순화대간기분을정.채용개량구씨법학정소서관위급약적LD50.이25%적농안수유발실험동물해、담,이실험동물적해수잠복기、2min내해수차수、기관분홍배담량위평개지표,관찰대간기분을정적진해、거담작용.결과:대간기분을정적반수치사량위288.3mg/kg.대간기분을정저、중、고제량조실험동물적해수잠복기분별위(16.67±5.70)s、(20.17±4.93)s、(23.45±6.53)s,균장우음성대조조실험동물적해수잠복기(14.53±3.86)s;여양성대조조상비,조간차이불현저.대간기분을정저、중、고제량조실험동물2min내해수차수분별위(34.77±11.47)차、(24.95±7.46)차、(26.25±7.68)차,균소우음성대조조(43.76±9.49)차,차여양성대조조상비,조간차이불현저.저、중、고제량대간기분을정균가제고담액분비량.결론:대간기분을정구유일정적독성차진해、거담작용명현.
Objective:To study the acute toxicity, antitussive and expectorant effects of p-hydroxyphenylaceto-nitrile.Methods:Separate the p-hydroxyphenylacetonitrile from roasted seeds of sinapis alba.Determine the security administration scope of p-hydroxyphenylacetonitrile by median lethal dose test;evoke the experimen-tal animal cough and expectoration symptoms by 25%ammonia;measure the cough latent period and count the cough times within 2 minutes.Measure the expectorant effect of p-hydroxyphenylacetonitrile by phenol red secretion from trachea expectoration test.Results:The median lethal dose of p-hydroxyphenylacetonitrile was 288.3mg/kg.P-hydroxyphenylacetonitrile could prolong the cough latent period, decrease cough times within 2 minutes and increase phenol red secretion from trachea in cough models induced by ammonia.Conclusion:After taking lethal dose p-hydroxyphenylacetonitrile, experimental animals take on the acute toxicosis symp-toms.The antitussive and expectorant effects are available after taking p-hydroxyphenylacetonitrile.