CAJ | 학술논문

目的:观察新血府逐瘀汤对AS大鼠主动脉病理形态学改变及转化生长因子-β(TGF-β)、血管细胞黏附分子-1(VCAM-1)表达的影响,探讨新血府逐瘀汤抗动脉粥样硬化(AS)的可能机制.方法:健康雄性Wistar大鼠40只,随机分为对照组,模型组,立普妥组,新血府逐瘀汤低剂量组及新血府逐瘀汤高剂量组.除空白组外,其他各组复制AS模型,中药低剂量组9g/(kg·d)、中药高剂量组18g/(kg·d)灌胃;立普妥组2mg/(kg·d)水溶液灌胃;对照组及模型组灌服等容积生理盐水,连续8周;HE染色镜下观察胸主动脉病理学改变,免疫组织化学法检测胸主动脉TGF-β、VCAM-1表达,并进行相关性分析.结果:与模型组比较,新血府逐瘀汤高剂量组可显著提高TGF-β表达(P<0.01),降低VCAM-1表达(P<0.05),两者存在显著负相关性(P<0.01).结论:新血府逐瘀汤在一定程度上可通过上调保护性因子TGF-β以抑制免疫炎症反应,发挥抗动脉粥样硬化的作用.
목적:관찰신혈부축어탕대AS대서주동맥병리형태학개변급전화생장인자-β(TGF-β)、혈관세포점부분자-1(VCAM-1)표체적영향,탐토신혈부축어탕항동맥죽양경화(AS)적가능궤제.방법:건강웅성Wistar대서40지,수궤분위대조조,모형조,립보타조,신혈부축어탕저제량조급신혈부축어탕고제량조.제공백조외,기타각조복제AS모형,중약저제량조9g/(kg·d)、중약고제량조18g/(kg·d)관위;립보타조2mg/(kg·d)수용액관위;대조조급모형조관복등용적생리염수,련속8주;HE염색경하관찰흉주동맥병이학개변,면역조직화학법검측흉주동맥TGF-β、VCAM-1표체,병진행상관성분석.결과:여모형조비교,신혈부축어탕고제량조가현저제고TGF-β표체(P<0.01),강저VCAM-1표체(P<0.05),량자존재현저부상관성(P<0.01).결론:신혈부축어탕재일정정도상가통과상조보호성인자TGF-β이억제면역염증반응,발휘항동맥죽양경화적작용.
Objective:To observe the effect of New Xuefu Zhuyu decoction (NXFZY) on aorta pathological changes in AS rats and the expressions of TGF-βand VCAM-1,and to explore the possible mechanism of anti AS.Methods:40 SPF male Wistar rats were randomly divided into five groups which were a blank control group,a model group,a Liptor group,a low dose NXFZY group and a high dose NXFZY group.Except the control group, other groups copied AS models.In low dose NXFZY and high dose NXFZY groups, rats were intragastrically administered by NXFZY at the concentration of 9g/( kg·d) and 18g/( kg·d) respectively. In the Liptor group, rats were intragastrically administered by an aqueous solution of Liptor 2mg/( kg·d) . Simultaneously, the control and model groups were intragastrically administered with equal volume of normal saline.After eight weeks,thoracic aortic pathology changes in rats were observed by light microscope with HE staining.TGF-βand VCAM-1 expressions of thoracic aortic pathology was detected by immunohistochemi-cal method, while the correlativity was analyzed.Results:Compared with the model group, the NXFZY could increase the expression of TGF-β(P<0.01) and decrease the expression of VCAM-1(P<0.05),and the two had a significantly negative correlation(P<0.01).Conclusion:NXFZY can inhibit the inflammatory re-sponse by upregulating protective factor TGF-βexpression, thus achieving the effect of anti-atherosclerosis.