CAJ | 학술논문

目的探究Zn(PMFPCl)2 对肝癌HepG2细胞的抑制作用及其机制.方法 HepG2细胞分组为对照组及实验组(10、30、70μmol/L).MTT法检测Zn(PMFPCl) 2 对肝癌HepG2细胞增殖的影响,流式细胞仪检测细胞凋亡及细胞周期,倒置显微镜下观察Zn(PMFPCl) 2 作用后肿瘤细胞的形态学变化,Western blot检测凋亡蛋白p53、p21、caspase-3、bax、bcl-2的表达情况.结果各实验组(10、30、70μmol/L)在24、48、72 h对细胞的抑制率均显著高于对照组(P<0.05).当Zn(PMFPCl)2 浓度70μmol/L,作用72 h时细胞的抑制率最高,达63.29%.各实验组在48 h时的细胞凋亡率显著高于对照组(P<0.05).Zn(PMFPCl)2 使细胞的生长停滞在G1期,阻碍细胞增殖.当Zn(PMFPCl) 2 作用细胞后贴壁细胞数量减少,细胞皱缩,细胞膜出现突起,细胞变圆、变亮.Western blot法检测p53、p21、caspase-3、bax随配合物浓度的增加而增加,而bcl-2随之减少(P<0.05).结论 Zn(PMFPCl)2 对肝癌HepG2细胞具有抑制增殖及促进凋亡的作用,其机制与促进p53、p21、caspase-3、bax表达而降低bcl-2表达有关.
목적 탐구Zn(PMFPCl)2 대간암HepG2세포적억제작용급기궤제.방법 HepG2세포분조위대조조급실험조(10、30、70μmol/L).MTT법검측Zn(PMFPCl) 2 대간암HepG2세포증식적영향,류식세포의검측세포조망급세포주기,도치현미경하관찰Zn(PMFPCl) 2 작용후종류세포적형태학변화,Western blot검측조망단백p53、p21、caspase-3、bax、bcl-2적표체정황.결과 각실험조(10、30、70μmol/L)재24、48、72 h대세포적억제솔균현저고우대조조(P<0.05).당Zn(PMFPCl)2 농도70μmol/L,작용72 h시세포적억제솔최고,체63.29%.각실험조재48 h시적세포조망솔현저고우대조조(P<0.05).Zn(PMFPCl)2 사세포적생장정체재G1기,조애세포증식.당Zn(PMFPCl) 2 작용세포후첩벽세포수량감소,세포추축,세포막출현돌기,세포변원、변량.Western blot법검측p53、p21、caspase-3、bax수배합물농도적증가이증가,이bcl-2수지감소(P<0.05).결론 Zn(PMFPCl)2 대간암HepG2세포구유억제증식급촉진조망적작용,기궤제여촉진p53、p21、caspase-3、bax표체이강저bcl-2표체유관.
Objective To explore the inhibition of Zn(PMFPCl) 2 on HepG2 cells and its mechanism.Methods The HepG2 cells were divided into control group and experimental group of 10, 30 and 70 μmol/L.The cell proliferation was detected by MTT assay, cell apoptosis and cell cycle was analysed by flow cytometry, cellular morphological change was observed with inverted microscope and the expressions of apoptosis-regulated proteins of p53, p21, caspase-3, bax and bcl-2 in HepG2 cells were detected by Western blot.Results The inhibitory rates of experimental groups (10, 30, 70μmol/L) at 24, 48 and 72h were significantly higher than those of control group (P<0.05), and the highest one was 63.29% of 70 μmol/L Zn (PMFPCl)2at 72 h.The apoptosis rates of each experimental group at 48h was significantly higher than that of control group (P<0.05).The cells were induced a remarkable G1 arrest by Zn(PMFPCl) 2 which could inhibit proliferation.The number of adherent cells reduced and cells shrank, convex on cytomembrane surface appeared and the cells changed to round and were brighter.Western blot results showed that the protein levels of p53, p21, caspase-3 and bax increased and bcl-2 decreased with the Zn(PMFPCl)2concentration increasing (P<0.05).Conclusion Zn(PMFPCl)2 could inhibit the proliferation and promote apoptosis of HepG2 cells whose mechanisms are promotation of p53, p21, caspase-3 and bax expressions and inhibition of bcl-2 expression.