CAJ | 학술논문

目的探讨补肾强精颗粒改善特发性弱精子症患者DNA完整性的临床效果.方法选取南昌市医学科学研究所附属医院男性不育门诊特发性弱精子症患者110例,采用随机数字表法将患者分为3组,治疗组(n=40)采用补肾强精颗粒治疗,对照组(n=40)采用五子衍宗丸治疗,空白对照组(n =30)给予安慰治疗,比较3 组间精液参数与精子 DNA 碎片化指数(DNA fragmentation index,DFI)的差异.结果治疗组治疗有效率明显高于对照组与空白对照组,对照组治疗有效率明显高于空白对照组,差异均具有统计学意义(P<0.05).治疗前后,3组间患者精液量、液化时间、a、a+b、a+b+c级精子百分率、精浆果糖与酸性磷酸酶水平比较差异无统计学意义.治疗前,3组间患者精子密度、精子畸形率、α-糖苷酶与DFI比较差异无统计学意义,治疗后,治疗组精子密度明显高于对照组与空白对照组,精子畸形率、DFI明显低于对照组与空白对照组,对照组DFI明显低于空白对照组,组间比较差异均有统计学意义(P<0.05).Pearson相关性分析显示,精子密度与DFI呈明显的负相关(r=-0.354),精子畸形率与DFI呈正相关(r=0.324).结论 DNA完整性异常的主要原因为肾功能失调,补肾强精颗粒可作为改善精子DNA完整性的有效药物.
목적 탐토보신강정과립개선특발성약정자증환자DNA완정성적림상효과.방법 선취남창시의학과학연구소부속의원남성불육문진특발성약정자증환자110례,채용수궤수자표법장환자분위3조,치료조(n=40)채용보신강정과립치료,대조조(n=40)채용오자연종환치료,공백대조조(n =30)급여안위치료,비교3 조간정액삼수여정자 DNA 쇄편화지수(DNA fragmentation index,DFI)적차이.결과 치료조치료유효솔명현고우대조조여공백대조조,대조조치료유효솔명현고우공백대조조,차이균구유통계학의의(P<0.05).치료전후,3조간환자정액량、액화시간、a、a+b、a+b+c급정자백분솔、정장과당여산성린산매수평비교차이무통계학의의.치료전,3조간환자정자밀도、정자기형솔、α-당감매여DFI비교차이무통계학의의,치료후,치료조정자밀도명현고우대조조여공백대조조,정자기형솔、DFI명현저우대조조여공백대조조,대조조DFI명현저우공백대조조,조간비교차이균유통계학의의(P<0.05).Pearson상관성분석현시,정자밀도여DFI정명현적부상관(r=-0.354),정자기형솔여DFI정정상관(r=0.324).결론 DNA완정성이상적주요원인위신공능실조,보신강정과립가작위개선정자DNA완정성적유효약물.
Objective To investigate clinical effect of kidney essence granule on DNA intergrity in patients with idiopathic weak sperm.Methods 110 cases of idiopathic weak sperm disease in outpatient service of male infertility were selected, and divided into 3 groups using random number table method, treatment group (n=40) treated with strong kidney essence granule and control group (n=40) by Wuzi Yanzong pill treatment, blank control group (n =30) given placebo treatment.Semen parameters and sperm DNA fragmentation index (DFI) differences were compared within groups. Results The efficacy of treatment group was significantly higher than control group and blank control group, and control group was significantly higher than blank control group, the differences were all statistically significant (P<0.05).There was no significant difference among the 3 groups of patients within semen volume, liquefaction time, a,(a+b),(a+b+c) grade sperm percentage, seminal plasma fructose and acid phosphatase level pre-and post-treatment.Pre-treatment, sperm density, sperm abnormality rate, α-glycosides and DFI were not significantly different among the 3 groups.Post-treatment, sperm density in treatment group was significantly higher than that in control group, and sperm deformity rate and DFI were significantly lower than that in control group, and that in control group was significantly lower than that in blank control group (P <0.05) .Pearson correlation analysis showed that the sperm density was negatively correlated with DFI(r=-0.354), and the sperm deformity rate was positively correlated with DFI(r=0.324).Conclusion The main reason for abnormal DNA integrity are renal dysfunction, and kidney essence granule can be used as an effective medicine to improve the integrity of sperm DNA.