中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
10期
18-21
,共4页
麻黄%内生真菌%麻黄类生物碱
痳黃%內生真菌%痳黃類生物堿
마황%내생진균%마황류생물감
ephedra%endophytic fungi%ephedra alkaloids
目的 从珍稀药用植物麻黄中分离内生真菌,检测其麻黄类生物碱产生情况,并探索最优发酵条件.方法 处理麻黄草质茎并分离内生真菌,碘化铋钾试剂初检生物碱,以麻黄碱、伪麻黄碱、甲基麻黄碱为对照采用HPLC法筛选产生麻黄类生物碱的菌株,以HPLC法检测总麻黄类生物碱含量并以其为评价指标筛选出碳源及氮源种类后,以碳源量、氮源量、pH值、温度、发酵时间5个考察因素设计L16 (45)进行正交试验,从而确定最佳发酵培养基和发酵条件.结果 麻黄草质茎中的内生真菌Es2发酵后菌丝体提取物含有麻黄碱、伪麻黄碱及甲基麻黄碱;Es3发酵后菌丝体提取物含有伪麻黄碱.乳糖和硫酸铵是Es2发酵产麻黄类生物碱最适宜的碳源和氮源,当碳源30 g/L,氮源4 g/L,pH5.0,温度30℃,发酵8 d时,Es产麻黄类生物碱的产量最高.结论 采用微生物发酵的方法实现麻黄类生物碱的工业化生产,可缓解麻黄碱市场需求对环境的压力.
目的 從珍稀藥用植物痳黃中分離內生真菌,檢測其痳黃類生物堿產生情況,併探索最優髮酵條件.方法 處理痳黃草質莖併分離內生真菌,碘化鉍鉀試劑初檢生物堿,以痳黃堿、偽痳黃堿、甲基痳黃堿為對照採用HPLC法篩選產生痳黃類生物堿的菌株,以HPLC法檢測總痳黃類生物堿含量併以其為評價指標篩選齣碳源及氮源種類後,以碳源量、氮源量、pH值、溫度、髮酵時間5箇攷察因素設計L16 (45)進行正交試驗,從而確定最佳髮酵培養基和髮酵條件.結果 痳黃草質莖中的內生真菌Es2髮酵後菌絲體提取物含有痳黃堿、偽痳黃堿及甲基痳黃堿;Es3髮酵後菌絲體提取物含有偽痳黃堿.乳糖和硫痠銨是Es2髮酵產痳黃類生物堿最適宜的碳源和氮源,噹碳源30 g/L,氮源4 g/L,pH5.0,溫度30℃,髮酵8 d時,Es產痳黃類生物堿的產量最高.結論 採用微生物髮酵的方法實現痳黃類生物堿的工業化生產,可緩解痳黃堿市場需求對環境的壓力.
목적 종진희약용식물마황중분리내생진균,검측기마황류생물감산생정황,병탐색최우발효조건.방법 처리마황초질경병분리내생진균,전화필갑시제초검생물감,이마황감、위마황감、갑기마황감위대조채용HPLC법사선산생마황류생물감적균주,이HPLC법검측총마황류생물감함량병이기위평개지표사선출탄원급담원충류후,이탄원량、담원량、pH치、온도、발효시간5개고찰인소설계L16 (45)진행정교시험,종이학정최가발효배양기화발효조건.결과 마황초질경중적내생진균Es2발효후균사체제취물함유마황감、위마황감급갑기마황감;Es3발효후균사체제취물함유위마황감.유당화류산안시Es2발효산마황류생물감최괄의적탄원화담원,당탄원30 g/L,담원4 g/L,pH5.0,온도30℃,발효8 d시,Es산마황류생물감적산량최고.결론 채용미생물발효적방법실현마황류생물감적공업화생산,가완해마황감시장수구대배경적압력.
Objective To isolate endophytic fungi from the rare medicinal plants ephedra, detect the production of ephedrine alkaloids, and explore the optimum fermentation conditions.Methods The endophytic fungi were isolated from the herbaceous stems treated.The ephedrine alkaloids were preliminarily detected by Dragendorff's reagent.The fungi strains producing ephedrine alkaloids were screened by HPLC method with reference substance of ephedrine, pseudoephedrine and methephedrine.After the optimum carbon source and nitrogen source were determined with total ephedrine alkaloids as evaluation indicators by HPLC, the orthogonal test of L16 (45) was designed to investigate five levels of carbon source amount, nitrogen source amount, pH value, temperature, fermentation time.Results The ephedra herbaceous stems endophytic fungus-Es2 mycelium extract after fermentation contained ephedrine, pseudoephedrine and methephedrine.Es3 mycelium extract containing pseudoephedrine after fermentation.Lactose and ammonium sulfate were as the most suitable carbon source and nitrogen source of Es2 fermentation to produce ephedrine alkaloids.Es produced the highest yield of ephedra alkaloids when the carbon source 30 g/L, nitrogen source 4 g/L, pH5.0, temperature 30℃, fermented for 8 days.Conclusion The ephedrine alkaloids of industrial production could be realized by microorganism fermentation method, which has very important significance to alleviate the ephedrine market demand pressure on the environment.
