CAJ | 학술논문

目的研究冬凌草对酒精性肝损伤模型大鼠炎性因子及肝功能的影响.方法选择SD大鼠120只,随机分为对照组、模型组以及治疗组,各40只,采用酒精灌胃的方式建立酒精性肝损伤模型,给予冬凌草治疗.采用电化学发光法检测血清中AST、ALT、α-GST、GLDH含量,荧光定量PCR检测肝脏组织中NF-κB、TNF-α、IL-6、IL-8的含量.结果模型组大鼠血清中AST、ALT、α-GST、GLDH含量及肝脏组织中NF-κB、TNF-α、IL-6、IL-8的含量均显著高于对照组(t=12.030~37.417, P<0.05);治疗组大鼠血清中AST、ALT、α-GST、GLDH含量及肝脏组织中NF-κB、TNF-α、IL-6、IL-8的含量均显著低于模型组(t=5.128~32.325, P<0.05).结论酒精灌胃能够造成肝损伤,冬凌草治疗有助于减轻肝损伤,缓解炎症反应.
목적 연구동릉초대주정성간손상모형대서염성인자급간공능적영향.방법 선택SD대서120지,수궤분위대조조、모형조이급치료조,각40지,채용주정관위적방식건립주정성간손상모형,급여동릉초치료.채용전화학발광법검측혈청중AST、ALT、α-GST、GLDH함량,형광정량PCR검측간장조직중NF-κB、TNF-α、IL-6、IL-8적함량.결과 모형조대서혈청중AST、ALT、α-GST、GLDH함량급간장조직중NF-κB、TNF-α、IL-6、IL-8적함량균현저고우대조조(t=12.030~37.417, P<0.05);치료조대서혈청중AST、ALT、α-GST、GLDH함량급간장조직중NF-κB、TNF-α、IL-6、IL-8적함량균현저저우모형조(t=5.128~32.325, P<0.05).결론 주정관위능구조성간손상,동릉초치료유조우감경간손상,완해염증반응.
Objective To study effect of rubescens on inflammatory cytokines and liver function of alcoholic liver injury rat model.Methods 120 SD rats were chosed for studied, randomly divided into control group, model group and treatment group,40 rats per group.Alcoholic liver injury rat model was made by ethanol feeding approach, rubescens were given for treatment.Then serum AST, ALT, α-GST, GLDH content and liver tissue NF-κB, TNF-α, IL-6, IL-8 levels were detected by electrochemiluminescence and fluorescent quantitation PCR,respectively.Results Model group serum AST, ALT,α-GST, GLDH contents and NF-κB, TNF-α, IL-6, IL-8 contents in liver tissue were higher than control group(t=12.030~37.417, P<0.05). Treatment group serum AST,ALT, α-GST, GLDH contents and NF-κB, TNF-α, IL-6, IL-8 contents in liver tissue were lower than model group (t=5.128~32.325, P<0.05).Conclusion Alcohol feeding can cause liver damage, rubescens therapy can reduce liver injury, relieve inflammation.