中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
10期
137-140
,共4页
池絮影%崔曰新%张蜀%邓红
池絮影%崔曰新%張蜀%鄧紅
지서영%최왈신%장촉%산홍
一测多评%腺苷%尿苷%鸟苷%(R,S)-告依春%UPLC%HPLC
一測多評%腺苷%尿苷%鳥苷%(R,S)-告依春%UPLC%HPLC
일측다평%선감%뇨감%조감%(R,S)-고의춘%UPLC%HPLC
QAMS%adenosine%uridine%guanosine%(R%S)-goitrin%UPLC%HPLC
目的 建立同时测定板蓝根颗粒中腺苷、尿苷、鸟苷、(R,S)-告依春的一测多评法.方法 以腺苷为内标物,确定板蓝根中其他3种成分相对于腺苷的校正因子,通过相对校正因子(relative correction factor,RCF)对尿苷、鸟苷、(R,S)-告依春进行定量,实现一测多评(计算法);同时采用外标法测定板蓝根颗粒中4种成分的含量(实测值),并比较一测多评法与外标法测定结果的差异.结果 各成分相对校正因子重现性良好.尿苷、鸟苷、(R,S)-告依春按一测多评方法与外标法测定结果差异无统计学意义.结论 本研究建立的一测多评法简便,测定结果准确可靠,可为板蓝根颗粒的质量控制提供参考.
目的 建立同時測定闆藍根顆粒中腺苷、尿苷、鳥苷、(R,S)-告依春的一測多評法.方法 以腺苷為內標物,確定闆藍根中其他3種成分相對于腺苷的校正因子,通過相對校正因子(relative correction factor,RCF)對尿苷、鳥苷、(R,S)-告依春進行定量,實現一測多評(計算法);同時採用外標法測定闆藍根顆粒中4種成分的含量(實測值),併比較一測多評法與外標法測定結果的差異.結果 各成分相對校正因子重現性良好.尿苷、鳥苷、(R,S)-告依春按一測多評方法與外標法測定結果差異無統計學意義.結論 本研究建立的一測多評法簡便,測定結果準確可靠,可為闆藍根顆粒的質量控製提供參攷.
목적 건립동시측정판람근과립중선감、뇨감、조감、(R,S)-고의춘적일측다평법.방법 이선감위내표물,학정판람근중기타3충성분상대우선감적교정인자,통과상대교정인자(relative correction factor,RCF)대뇨감、조감、(R,S)-고의춘진행정량,실현일측다평(계산법);동시채용외표법측정판람근과립중4충성분적함량(실측치),병비교일측다평법여외표법측정결과적차이.결과 각성분상대교정인자중현성량호.뇨감、조감、(R,S)-고의춘안일측다평방법여외표법측정결과차이무통계학의의.결론 본연구건립적일측다평법간편,측정결과준학가고,가위판람근과립적질량공제제공삼고.
Objective To establish a quantitative analysis of multi-components by single-marker (QAMS) for determination of four components (adenosine, uridine, guanosine, (R, S)-goitrin) in Isatidis Radix granules.Method The QAMS for Isatidis Radix granules was established and validated and adenosine was selected as the internal reference substance.The relative correction factor (RCF) of other three components were calculated.The contents of four components were determined by both external standard method and QAMS.The QAMS method was validated through comparison of the results obtained by the two different methods.Results The RCFs of uridine, guanosine and (R, S)-goitrin all showed good reproducibility within certain ranges.For Isatidis Radix granules, uridine, guanosine and (R, S)-goitrin, there was no significant difference between the quantitative results of the two methods.Conclusions The QAMS method is simple, feasible and credible to be used for comprehensive quality control of Isatidis Radix granules.
