中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
10期
131-133
,共3页
高效凝胶色谱法%麦芽糖铁糖浆%分子量%分子量分布
高效凝膠色譜法%麥芽糖鐵糖漿%分子量%分子量分佈
고효응효색보법%맥아당철당장%분자량%분자량분포
HPGPC%Iron Maltose Syrup%molecular weight%molecular weight distribution
目的 建立测定麦芽糖铁糖浆的分子量与分子量分布的方法.方法 采用高效凝胶色谱法(high performance gel permeation chromatography,HPGPC) ,色谱柱为PSS HEMA凝胶色谱柱,检测器为示差折光检测器,流动相为磷酸盐缓冲液(pH6.8) ,柱温为45℃,流速为0.5 mL/min.结果 3批样品的重均分子量分布在45000~47000 Da之间,精密度、重现性好.结论 该方法简单,重现性好,可以作为控制麦芽糖铁糖浆中分子量与分子量分布的方法.
目的 建立測定麥芽糖鐵糖漿的分子量與分子量分佈的方法.方法 採用高效凝膠色譜法(high performance gel permeation chromatography,HPGPC) ,色譜柱為PSS HEMA凝膠色譜柱,檢測器為示差摺光檢測器,流動相為燐痠鹽緩遲液(pH6.8) ,柱溫為45℃,流速為0.5 mL/min.結果 3批樣品的重均分子量分佈在45000~47000 Da之間,精密度、重現性好.結論 該方法簡單,重現性好,可以作為控製麥芽糖鐵糖漿中分子量與分子量分佈的方法.
목적 건립측정맥아당철당장적분자량여분자량분포적방법.방법 채용고효응효색보법(high performance gel permeation chromatography,HPGPC) ,색보주위PSS HEMA응효색보주,검측기위시차절광검측기,류동상위린산염완충액(pH6.8) ,주온위45℃,류속위0.5 mL/min.결과 3비양품적중균분자량분포재45000~47000 Da지간,정밀도、중현성호.결론 해방법간단,중현성호,가이작위공제맥아당철당장중분자량여분자량분포적방법.
Objective To establish a method for determining the molecular weight (Mw) and molecular weight distribution of Iron Maltose Syrup. Methods HPGPC was used; PSS HEMA was used as column.Detector was differential refraction detector.Mobile phase was phosphate buffer solution (pH6.8) at 0.5 mL/min, column temperature was 45℃.Results The Mw of 3 batches of Iron Maltose Syrup were 45000-47000 Da with good linearity, precision and reproducibility.Conclusion The method is simple, reliable and accepted by the specification for controlling the molecular weight and weight distribution of Iron Maltose Syrup.
