中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
10期
144-146
,共3页
蒋丽萍%刘国强%徐宏祥%傅应华
蔣麗萍%劉國彊%徐宏祥%傅應華
장려평%류국강%서굉상%부응화
毛细管气相色谱法%炒艾叶%艾叶炭%桉油精%含量测定%质量考察
毛細管氣相色譜法%炒艾葉%艾葉炭%桉油精%含量測定%質量攷察
모세관기상색보법%초애협%애협탄%안유정%함량측정%질량고찰
capillary gas chromatography%chao aiye%aiye tan%eucalyptol%content determination%quality inspection
目的 为了提高艾叶炮制品质量,对市售艾叶2个炮制品炒艾叶与艾叶炭中桉油精含量进行考察.方法 采用毛细管气相色谱法,用正已烷回流提取样品;色谱条件:以Agilent DB-1甲基硅橡胶石英毛细管柱(30 m ×0.320 mm ×0.25μm)为色谱柱,进样口温度200℃,氢火焰离子化检测器(flame ionization detector, FID)温度250℃;程序升温,起始温度为100℃,保持6 min,再以20℃/min的速率升至160℃,保持3 min;分流进样,分流比5.0:1;载气为氮气,流速1.0 mL/min;进样量1μL.结果 在给定的色谱条件下,桉油精分离良好,与其它杂质峰分离度大于1.5;桉油精在11.4~114.0μg/mL之间与峰面积呈良好的线性关系,方法重复性和加样回收率均好,最小定量限0.5μg/mL.通过对桉油精含量测定,表明市售9家中药饮片厂生产的11批炒艾叶中桉油精含量在5.6~78.2μg/g之间,12批艾叶炭桉油精含量均未检出.结论 目前市售艾叶炮制品炒艾叶、艾叶炭的炮制工艺有待进一步研究改进,需要提高质量标准.
目的 為瞭提高艾葉砲製品質量,對市售艾葉2箇砲製品炒艾葉與艾葉炭中桉油精含量進行攷察.方法 採用毛細管氣相色譜法,用正已烷迴流提取樣品;色譜條件:以Agilent DB-1甲基硅橡膠石英毛細管柱(30 m ×0.320 mm ×0.25μm)為色譜柱,進樣口溫度200℃,氫火燄離子化檢測器(flame ionization detector, FID)溫度250℃;程序升溫,起始溫度為100℃,保持6 min,再以20℃/min的速率升至160℃,保持3 min;分流進樣,分流比5.0:1;載氣為氮氣,流速1.0 mL/min;進樣量1μL.結果 在給定的色譜條件下,桉油精分離良好,與其它雜質峰分離度大于1.5;桉油精在11.4~114.0μg/mL之間與峰麵積呈良好的線性關繫,方法重複性和加樣迴收率均好,最小定量限0.5μg/mL.通過對桉油精含量測定,錶明市售9傢中藥飲片廠生產的11批炒艾葉中桉油精含量在5.6~78.2μg/g之間,12批艾葉炭桉油精含量均未檢齣.結論 目前市售艾葉砲製品炒艾葉、艾葉炭的砲製工藝有待進一步研究改進,需要提高質量標準.
목적 위료제고애협포제품질량,대시수애협2개포제품초애협여애협탄중안유정함량진행고찰.방법 채용모세관기상색보법,용정이완회류제취양품;색보조건:이Agilent DB-1갑기규상효석영모세관주(30 m ×0.320 mm ×0.25μm)위색보주,진양구온도200℃,경화염리자화검측기(flame ionization detector, FID)온도250℃;정서승온,기시온도위100℃,보지6 min,재이20℃/min적속솔승지160℃,보지3 min;분류진양,분류비5.0:1;재기위담기,류속1.0 mL/min;진양량1μL.결과 재급정적색보조건하,안유정분리량호,여기타잡질봉분리도대우1.5;안유정재11.4~114.0μg/mL지간여봉면적정량호적선성관계,방법중복성화가양회수솔균호,최소정량한0.5μg/mL.통과대안유정함량측정,표명시수9가중약음편엄생산적11비초애협중안유정함량재5.6~78.2μg/g지간,12비애협탄안유정함량균미검출.결론 목전시수애협포제품초애협、애협탄적포제공예유대진일보연구개진,수요제고질량표준.
Objective To improve the quality of aiye processed products, an eucalyptol content in commercially available aiye two processed products of chao aiye and aiye tan was investigated.Methods A capillary gas chromatography was used.The sample was prepared with n-hexane by reflux condensation.Chromatographic conditions: The separation was carried on an Ailgent DB-1 capillary column(30 mm ×0.320 mm ×0.25 μm). Inlet temperature was 200℃ and FID temperature was 250℃.The programmed column temperature was set as follows:maintained at 100℃ for 6 min and raised to 160℃ at the rate of 20℃/min followed by holding for 3min.The splitting-ratio was 5.0:1.The carried gas was nitrogen, flow rate was 1.0 mL/min.Injection volume was 1μL.Results In the given chromatographic conditions, the eucalyptol chromatographic separation had good, and the separation degree was greater than 1.5 between eucalyptol and other impurity peak.The linear range of eucalyptol was 11.4-114.0 mg/mL(r=0.999 5). Methods repeatability and recovery were good.The minimum limit of quantification was 0.5μg/mL.The results of determination of eucalyptol show that the eucalyptol content in the commercially available 11 batch of chao aiye was between 5.6-78.2 μg /g, and 12 batch of aiye tan had no eucalyptol. Conclusion The processing technology of current commercially available aiye processed products of chao aiye and aiye tan need to be improved, and the quality standard need to be improved.