中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
Chinese Journal of Biochemical Pharmaceutics
2015年
10期
141-143
,共3页
厄贝沙坦%含量测定%高效液相色谱法
阨貝沙坦%含量測定%高效液相色譜法
액패사탄%함량측정%고효액상색보법
irbesartan%content determination%HPLC
目的 建立测定药物中厄贝沙坦含量的高效液相色谱法.方法 采用Diamonsil C18柱(150 mm ×4.6 mm,5μm),以磷酸溶液(以三乙胺调节pH至3.2)-乙腈(62:38)为流动相,在柱温35℃,流速约1.0 mL/min,检测波长245 nm的条件下进行检测.结果 厄贝沙坦的质量浓度在0.04~0.40 mg/mL范围内与峰面积线性关系良好(r=0.99999),平均加样回收率为98.37%,RSD为0.7%(n=9).结论 该方法操作简便、定量准确、线性响应范围宽,重复性好,可作为药物中厄贝沙坦含量测定的方法.
目的 建立測定藥物中阨貝沙坦含量的高效液相色譜法.方法 採用Diamonsil C18柱(150 mm ×4.6 mm,5μm),以燐痠溶液(以三乙胺調節pH至3.2)-乙腈(62:38)為流動相,在柱溫35℃,流速約1.0 mL/min,檢測波長245 nm的條件下進行檢測.結果 阨貝沙坦的質量濃度在0.04~0.40 mg/mL範圍內與峰麵積線性關繫良好(r=0.99999),平均加樣迴收率為98.37%,RSD為0.7%(n=9).結論 該方法操作簡便、定量準確、線性響應範圍寬,重複性好,可作為藥物中阨貝沙坦含量測定的方法.
목적 건립측정약물중액패사탄함량적고효액상색보법.방법 채용Diamonsil C18주(150 mm ×4.6 mm,5μm),이린산용액(이삼을알조절pH지3.2)-을정(62:38)위류동상,재주온35℃,류속약1.0 mL/min,검측파장245 nm적조건하진행검측.결과 액패사탄적질량농도재0.04~0.40 mg/mL범위내여봉면적선성관계량호(r=0.99999),평균가양회수솔위98.37%,RSD위0.7%(n=9).결론 해방법조작간편、정량준학、선성향응범위관,중복성호,가작위약물중액패사탄함량측정적방법.
Objective To establish a HPLC method for the content determination of irbesartan in drugs.Methods The diamonsil C18 column (150 mm ×4.6 mm,5μm) was used, with the mobile phase of phosphoric acid solution-acetonitrile(adjusted to pH 3.2 with triethylamine)(62:38).The column temperature was 35℃, with the UV detection wavelength of 245 nm and a flow rate of 1.0 mL/min.Results Irbesartan exhibited a good linear relationship with the peak area in the concentration range of 0.04-0.40 mg/mL with the correlation coefficient of 0.999 99.The average recovery was 98.37% with RSD of 0.7%(n=9).Conclusion This method is simple and accurate with wide linear range and good reproducibility,and can be used to determine the content of irbesartan in drugs.
