CAJ | 학술논문

目的:本文旨在研究胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)D442G基因多态性与脑梗死发病的相关性. 方法:选取包头医学院第一附属医院神经内科脑梗死患者180例为观察组,体检科体检人员130例为对照组. DNA试剂盒提取外周血DNA,采用聚合酶链反应-限制性片段长度多态性( polymerase chain reaction-restriction fragment length poly-morph,PCR-RFLP)方法检测CETP D442G的基因型,分析D442G基因型与脑梗死发病的关系. 结果:D442G基因位点DG基因型高密度脂蛋白水平高于GG、DD基因型,差异有统计学意义( P <0. 05). 以是否发生脑梗死作为应变量,以年龄、性别、体重指数、血脂水平、糖尿病、高血压、心脏病、CETP D442G基因型及等位基因频率作为自变量,应用非条件logistic回归分析得出年龄、高血压、糖尿病、甘油三酯、胆固醇、低密度脂蛋白为脑梗死发病的独立危险因素( OR >1, P <0. 05);高密度脂蛋白、DG基因型为脑梗死发病的保护因素( OR <1, P <0. 05). 结论:脑梗死发病的危险因素可能为年龄、吸烟史、高血压病史、糖尿病病史、胆固醇、低密度脂蛋白、甘油三酯水平,脑梗死发病的保护因素可能为D442G基因DG基因型及高密度脂蛋白水平.
목적:본문지재연구담고순지전운단백(cholesteryl ester transfer protein,CETP)D442G기인다태성여뇌경사발병적상관성. 방법:선취포두의학원제일부속의원신경내과뇌경사환자180례위관찰조,체검과체검인원130례위대조조. DNA시제합제취외주혈DNA,채용취합매련반응-한제성편단장도다태성( polymerase chain reaction-restriction fragment length poly-morph,PCR-RFLP)방법검측CETP D442G적기인형,분석D442G기인형여뇌경사발병적관계. 결과:D442G기인위점DG기인형고밀도지단백수평고우GG、DD기인형,차이유통계학의의( P <0. 05). 이시부발생뇌경사작위응변량,이년령、성별、체중지수、혈지수평、당뇨병、고혈압、심장병、CETP D442G기인형급등위기인빈솔작위자변량,응용비조건logistic회귀분석득출년령、고혈압、당뇨병、감유삼지、담고순、저밀도지단백위뇌경사발병적독립위험인소( OR >1, P <0. 05);고밀도지단백、DG기인형위뇌경사발병적보호인소( OR <1, P <0. 05). 결론:뇌경사발병적위험인소가능위년령、흡연사、고혈압병사、당뇨병병사、담고순、저밀도지단백、감유삼지수평,뇌경사발병적보호인소가능위D442G기인DG기인형급고밀도지단백수평.
Objective:To study the relationship between cholesteryl ester transfer protein D442G polymorphism and cerebral infarc-tion. Methods:180 patients with cerebral infarction hospitalized in Neurology Department of the First Affiliated Hospital of Baotou Medical College were selected as the observation group and 130 healthy personnel in the Physical Examination Department as the control group. DNA was extracted from peripheral blood and the genotype of CETP D442G was detected by polymerase chain reaction-restriction frag-ment length polymorph (PCR-RFLP) method, the relationship between the genotype of D442G and cerebral infarction was analyzed. Re-sults:The high-density lipoprotein level of the DG genotype at the D442G locus was significantly higher than that of GG and DD geno-type, the difference being significant ( P <0. 05). Whether cerebral infarction would occur was used as a dependent variable and age, gender, body mass index, blood lipid levels, diabetes, hypertension, heart disease, the genotype of CETP D442G and allele frequency were used as variables;non-conditional logistic regression analysis was used to get the results that age, hypertension, diabetes, triglycer-ide, cholesterol and low density lipoprotein were the independent risk factors for the incidence of cerebral infarction( OR >1, P <0. 05) while high density lipoprotein and the DG genotype were the protective factors for the incidence of cerebral infarction ( OR <1, P <0. 05 ) . Conclusion:The independent risk factors for the incidence of cerebral infarction age, history of smoking, hypertension and diabetes, triglyceride levels, cholesterol and low density lipoprotein and the protective factors for the incidence of cerebral infarction may be high density lipoprotein and the DG genotype of D442G.